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HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003)

Publications (892) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:HRP-conjugated Goat anti-Mouse IgG (H+L)
ABclonal:Western blot - HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003)

Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) antibody (AS003) at 1:5000/1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameHRP-conjugated Goat anti-Mouse IgG (H+L)
Catalog No.AS003
Host speciesGoat
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • DB 1:2000 - 1:10000
  • ELISA 1:5000 - 1:10000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples
Cellular location
Customer validation

WB(Drosophila, Spodoptera frugiperda, Mus musculus, Rattus norvegicus, Crassostrea gigas, Homo sapiens, Brachyura, Caenorhabditis Elegans, Sus scrofa, Litopenaeus vannamei, Oryctolagus cuniculus, Gallus gallus, Malus micromalus, Tegillarca granosa, Drosophila melanogaster, Ostreidae, Tree, Flowers, Toxoplasma gondii, Oryza sativa, Bos taurus, Ictalurus punctatus, Chlorocebus aethiops , Cyprinus carpio, Arabidopsis thaliana, Escherichia coli, Plutella xylostella, Danio rerio, Portunus trituberculatus, Mesocricetus auratus, Carassius auratus gibelio, Bombyx mori Linnaeus, Gossypium spp, Mus musculus、Sus scrofa, Ctenopharyngodon idellus, Sinonovacula constricta, Solanum tuberosum , Ostrinia furnacalis , Capra hircus, Sus domesticus, A. fumigatus, Pyropia yezoensis, Saccharomyces cerevisiae, Duck plague virus, Lycopersicon esculentum, Ovis aries, Triticum aestivum, Lactococcus, Nicotiana tabacum, Aedes albopictus, Oreochromis niloticus, Zea mays, Cricetulus griseus, Cricetulus migratorius, Ark clam, African swine fever virus, Spodoptera frugiperdaMus musculus , Yellow catfish, Lacticaseibacillus case, Rattus norvegicus, Mus musculus, Pimephales promelas, Other, A. fumigatus strains, Stemphylium eturmiunum, Cabbage beetles, Pseudomonas putida, Macaca fascicularis, Bos taurus, Danio rerio, Trifoliata orange, Felis catus, Glycine max, Schizothorax prenanti, Canis familiaris, Chlorocebus aethiops)

ELISA(Crassostrea gigas, Sus scrofa, Mus musculus, Homo sapiens, Ctenopharyngodon idellus, Chiloscyllium plagiosum, Homo sapiens, Other, Toxoplasma gondii)

(Homo sapiens)

(Homo sapiens)

IHC(Mus musculus, Rattus norvegicus, Cervus nippon, Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Mus musculus, Homo sapiens, Mus musculus)

IP(Mus musculus, Triticum aestivum, N. benthamiana)

ELISA(Mus musculus, Homo sapiens)

WB(Homo sapiens)

IHC(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Homo sapiens, Mus musculus, Homo sapiens, Mus musculus, Rattus norvegicus)

RT-PCR(Mus musculus, Homo sapiens, Mus musculus)

IP(Homo sapiens , Nicotiana tabacum, Spodoptera frugiperda)

Co-IP(Other, Rattus norvegicus, Oryza sativa, Mus musculus, Homo sapiens)

ChIP(Homo sapiens)

Pull-down(Oryza sativa)

Pull-down(Oryza sativa)

Co-IP(Homo sapiens, Mus musculus)

RIP(Rattus norvegicus)

WB(Homo sapiens, Mus musculus, Other)

Other(Rattus norvegicus, Homo sapiens, Mus musculus)

RT-qPCR(Mus musculus)

IP(Homo sapiens)

Co-IP(Homo sapiens)

FC(Homo sapiens)

IF(Homo sapiens)

RT-qPCR(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003)}

    Western blot - HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003)

    Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody.
    Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) antibody (AS003) at 1:5000/1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS003? Please let us know so that we can cite the reference in this datasheet.