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Recombinant Human sTfR/CD71 Protein (RP00085)

Bioactivityvalidated

Publication (1) Datasheet

ABclonal:Recombinant Human sTfR/CD71 Protein

Overview

Product nameRecombinant Human sTfR/CD71 Protein
Catalog No.RP00085
DescriptionRecombinant Human sTfR/CD71 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Cys89-Phe760) of human sTfR (Accession #NP_001121620.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized Recombinant human Transferrin at 2 μg/mL (100 μL/well) can bind Recombinant human TfR with a linear range of 4-17 ng/mL.
Purity> 97% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW76.01 kDa
This protein is a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.
SynonymTFRC; CD71; IMD46; T9; TFR; TFR1; TR; TRFR; p90; transferrin receptor; CD71; IMD46; T9; TFR; TFR1; TR; TRFR; p90
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RP00085? Please let us know so that we can cite the reference in this datasheet.

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