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Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. HeLa cells were treated by TNF-α (50 ng/ml) and Calyculin A (50 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from NIH/3T3 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. NIH/3T3 cells were treated by TNF-α (10 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from C6 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at 1:10000 dilution. C6 cells were treated by TNF-α (50 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Immunofluorescence analysis of HeLa TNFα + CA and HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	Phospho-NF-kB p65/RelA-S536 Rabbit pAb
Catalog No.	AP0124
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S536 of human RelA (NP_068810.3).
Sequence	FSSIA
Gene ID
Swiss Prot
Synonyms	p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S536
Calculated MW	60kDa
Observed MW	65kDa/

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa treated by TNF-α and Calyculin A, NIH/3T3 treated by TNF-α, C6 treated by TNF-α, NIH/3T3, C6
Cellular location	Cytoplasm, Nucleus.
Customer validation	(CAL27) WB(Homo sapiens, Mus musculus, Rattus norvegicus, King Charles Spaniel, Sus scrofa, Other) IHC(Mus musculus) IHC(Rattus norvegicus, Mus musculus) ELISA(Mus musculus) WB(Mus musculus) IF(Mus musculus)

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Research Area

Phospho-NF-kB p65/RelA-S536 Rabbit pAb images

Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Immunofluorescence - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Molecular immunology

Application:

IF:

4.407

Species:

Homo sapiens

PMID:

28073078

Title:

TRIM52: A nuclear TRIM protein that positively regulates the nuclear factor-kappa B signaling pathway

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Biomed Res Int

Application:

IF:

3.411

Species:

Homo sapiens

PMID:

32714980

Title:

CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Biol Res

Application:

IF:

5.612

Species:

Homo sapiens

PMID:

32958054

Title:

REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

J Affect Disord

Application:

IF:

4.839

Species:

Mus musculus

PMID:

32553389

Title:

EZH2 is involved in vulnerability to neuroinflammation and depression-like behaviors induced by chronic stress in different aged mice

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Cell Death Discov

Application:

IF:

5.241

Species:

Homo sapiens

PMID:

33597510

Title:

Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Journal of physiology and biochemistry

Application:

IF:

Species:

Homo sapiens

PMID:

33411212

Title:

MicroRNA-520c-3p suppresses vascular endothelium dysfunction by targeting RELA and regulating the AKT and NF-κB signaling pathways

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.29

Species:

Homo sapiens

PMID:

35770335

Title:

Annexin-1 is an oncogene in glioblastoma and causes tumour immune escape through the indirect upregulation of interleukin-8 - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Frontiers in cellular and infection microbiology

Application:

IF:

6.07

Species:

Rattus norvegicus

PMID:

35811665

Title:

Gut Microbiota-Derived Diaminopimelic Acid Promotes the NOD1/RIP2 Signaling Pathway and Plays a Key Role in the Progression of Severe Acute Pancreatitis - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Brain research

Application:

IF:

3.61

Species:

Mus musculus

PMID:

36731819

Title:

Icariin Ameliorates Behavioral Deficits and Neuropathology in a Mouse Model of Multiple Sclerosis

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Drug design, development and therapy

Application:

IHC

IF:

4.31

Species:

Mus musculus

PMID:

36866196

Title:

Effects and Potential Mechanism of Zhuyu Pill Against Atherosclerosis: Network Pharmacology and Experimental Validation - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Mus musculus

PMID:

36923354

Title:

Niaoduqing alleviates podocyte injury in high glucose model via regulating multiple targets and AGE/RAGE pathway: Network pharmacology and experimental validation - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Journal of natural products

Application:

IF:

4.8

Species:

Mus musculus

PMID:

37002536

Title:

Synthesis of Lathyrol PROTACs and Evaluation of Their Anti-Inflammatory Activities

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Phytochemistry

Application:

IF:

Species:

Mus musculus

PMID:

36933878

Title:

Ent-kauranes and ent-atisanes from Euphorbia wallichii and their anti-inflammatory activity

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

6.37

Species:

Homo sapiens

PMID:

37605109

Title:

AGEs promote atherosclerosis by increasing LDL transcytosis across endothelial cells via RAGE/NF-κB/Caveolin-1 pathway - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Mus musculus

PMID:

37979447

Title:

CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-B pathway - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Aging

Application:

IF:

5.95

Species:

Homo sapiens

PMID:

37589506

Title:

TNFR2 promotes pancreatic cancer proliferation, migration, and invasion via the NF-B signaling pathway - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

King Charles Spaniel

PMID:

38056143

Title:

Exploration of the mechanisms of Callicarpa nudiflora Hook. et Arn against influenza A virus (H1N1) infection

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

The American journal of Chinese medicine

Application:

IF:

Species:

Rattus norvegicus

PMID:

37865871

Title:

Pachymic Acid Prevents Hemorrhagic Shock-Induced Cardiac Injury by Suppressing M1 Macrophage Polarization and NF-B Signaling Pathway

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

IF:

8.02

Species:

Homo sapiens

PMID:

36252620

Title:

CRM197-conjugated multi antigen dominant epitope for effective human cytomegalovirus vaccine development

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Biological & pharmaceutical bulletin

Application:

IF:

2.26

Species:

Rattus norvegicus

PMID:

38057100

Title:

Tanshinone IIA alleviates early brain injury after subarachnoid hemorrhage in rats by inhibiting the activation of NF-B/NLRP3 inflammasome

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Biological & pharmaceutical bulletin

Application:

IF:

2.26

Species:

Rattus norvegicus

PMID:

38057100

Title:

Tanshinone IIA alleviates early brain injury after subarachnoid hemorrhage in rats by inhibiting the activation of NF-B/NLRP3 inflammasome

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Journal of immunotoxicology

Application:

IF:

3.43

Species:

Homo sapiens

PMID:

37417782

Title:

Endoplasmic reticulum stress mediates environmental particle-induced inflammatory response in bronchial epithelium

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Inflammation

Application:

IF:

4.65

Species:

Mus musculus

PMID:

38294580

Title:

Fecal Microbiota Transplantation Alleviates Allergic Rhinitis via CD4+ T Cell Modulation Through Gut Microbiota Restoration

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Bioorganic chemistry

Application:

IF:

5.3

Species:

Mus musculus

PMID:

38367429

Title:

Diterpenoids from Euphorbia peplus possessing cytotoxic and anti-inflammatory activities

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF:

5.8

Species:

Rattus norvegicus

PMID:

38408681

Title:

CGRP attenuates pulmonary vascular remodeling by inhibiting the cGAS-STING-NFB pathway in pulmonary arterial hypertension

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Vet Microbiol

Application:

IF:

3.246

Species:

Sus scrofa

PMID:

34411995

Title:

Deletion of the crp gene affects the virulence and the activation of the NF-B and MAPK signaling pathways in PK-15 and iPAM cells derived from G. parasuis serovar 5

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Pharmacological Research

Application:

IF:

10.334

Species:

Mus musculus

PMID:

29391237

Title:

Sulforaphane attenuates microglia-mediated neuronal necroptosis through down-regulation of MAPK/NF-B signaling pathways in LPS-activated BV-2 microglia

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Int J Biol Sci

Application:

WB, IHC

IF:

10.75

Species:

Rattus norvegicus

PMID:

33867854

Title:

Moderate mechanical stimulation rescues degenerative annulus fibrosus by suppressing caveolin-1 mediated pro-inflammatory signaling pathway

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

ELISA, WB, IHC

IF:

6.2

Species:

Mus musculus

PMID:

37569344

Title:

Aloe-Emodin Ameliorates Cecal Ligation and Puncture-Induced Sepsis

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Journal of neurosurgical anesthesiology

Application:

IF:

3.7

Species:

Mus musculus

PMID:

38577840

Title:

Morphine Preconditioning Alleviates Ischemia/Reperfusion-induced Caspase-8dependent Neuronal Apoptosis through cPKC-NF-B-cFLIPL Pathway

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Cells

Application:

IF:

7.66

Species:

Sus scrofa

PMID:

36611975

Title:

CXCR3 Inhibition Blocks the NF-B Signaling Pathway by Elevating Autophagy to Ameliorate Lipopolysaccharide-Induced Intestinal Dysfunction in Mice - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Phytochemistry

Application:

IF:

4.004

Species:

Other

PMID:

34628107

Title:

Diterpenoid alkaloids from Delphinium forrestii var. viride and their anti-inflammation activity

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Food chemistry

Application:

IF:

8.8

Species:

Mus musculus

PMID:

38870817

Title:

Flavonoid-rich sesame leaf extract-mediated synthesis of nanozymes: Extraction optimization, chemical composition identification and bioactivity evaluation

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Translational andrology and urology

Application:

IF:

1.9

Species:

Mus musculus

PMID:

39434759

Title:

NOSTRIN is involved in benign prostatic hyperplasia via inhibition of proliferation, oxidative stress, and inflammation in prostate epithelial cells - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0124)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit pAb

Journal:

Gut microbes

Application:

IF:

12.2

Species:

Mus musculus

PMID:

39410876

Title:

High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis - PMC