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Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294) at1:1000 dilution (upper) or [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331) at1:50000 dilution (lower) incubated overnight at 4℃. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by TNF-α (20 ng/ml) and Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from NIH/3T3 cells using Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294) at 1:19000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from C6 cells using Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294) at 1:19000 dilution incubated at room temperature for 1.5 hours. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	Phospho-NF-kB p65/RelA-S536 Rabbit mAb
Catalog No.	AP1294
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55585

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S536 of human Phospho-NF-kB p65/RelA-S536 (NP_068810.3).
Sequence	FSSIA
Gene ID
Swiss Prot
Synonyms	p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S536
Calculated MW	60kDa
Observed MW	65kDa/

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:10000 - 1:20000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by TNF-α, HeLa treated by Calyculin A, C6 treated by TNF-α and Calyculin A, HeLa, C6
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens, Drosophila melanogaster)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-NF-kB p65/RelA-S536 Rabbit mAb images

Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Bioactive materials

Application:

IF:

16.87

Species:

Mus musculus

PMID:

38318229

Title:

Enzyme-like biomimetic oral-agent enabling modulating gut microbiota and restoring redox homeostasis to treat inflammatory bowel disease - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Acta biomaterialia

Application:

IF:

9.7

Species:

Mus musculus

PMID:

38458511

Title:

Tumor-targeted Delivery of Copper-manganese Biomineralized Oncolytic Adenovirus for Colorectal Cancer Immunotherapy

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.3

Species:

Rattus norvegicus

PMID:

38693854

Title:

Improved therapeutic effects on vascular intimal hyperplasia by mesenchymal stem cells expressing MIR155HG that function as a ceRNA for microRNA-205 - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

38720773

Title:

Ganoderma lucidum spore extract improves sleep disturbances in a rat model of sporadic Alzheimers disease - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Journal of medicinal chemistry

Application:

IF:

7.3

Species:

Homo sapiens

PMID:

38498304

Title:

Design and Synthesis of 3-(2H-Chromen-3-yl)-5-aryl-1,2,4-oxadiazole Derivatives as Novel Toll-like Receptor 2/1 Agonists That Inhibit Lung Cancer In Vitro and In

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Aging cell

Application:

IF:

7.8

Species:

Homo sapiens

PMID:

38482753

Title:

Mitophagy defect mediates the agingassociated hallmarks in HutchinsonGilford progeria syndrome

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Molecular nutrition & food research

Application:

IF:

5.2

Species:

Mus musculus

PMID:

38778506

Title:

Dietary Oat Glucan Alleviates HighFat Induced Insulin Resistance through Regulating Circadian Clock and Gut Microbiome

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Biomaterials research

Application:

IF:

11.3

Species:

Homo sapiens

PMID:

39011520

Title:

Genetically Modified Hepatocytes Targeting Bilirubin and Ammonia Metabolism for the Construction of Bioartificial Liver System - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Nucleic acids research

Application:

IF:

14.9

Species:

Drosophila melanogaster

PMID:

38742642

Title:

NF-B factors cooperate with Su(Hw)/E4F1 to balance Drosophila/human immune responses via modulating dynamic expression of miR-210

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Journal of inflammation research

Application:

IF:

4.5

Species:

Mus musculus

PMID:

39380664

Title:

Mangiferin Ameliorates CCl4-Triggered Acute Liver Injury by Inhibiting Inflammatory Response and Oxidative Stress: Involving the Nrf2-ARE Pathway - PMC

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

Journal of virology

Application:

IF:

5.4

Species:

Homo sapiens

PMID:

38775480

Title:

GRP78 recognizes EV-F 3D protein and activates NF-B to repress virus replication by interacting with CHUK/IKBKB

References for Phospho-NF-kB p65/RelA-S536 Rabbit mAb (AP1294)

Product:

Phospho-NF-kB p65/RelA-S536 Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38442575

Title:

Identification of Treg-related prognostic molecular subtypes and individualized characteristics in clear cell renal cell carcinoma through single-cell transcriptomes and