Overview
Product name | Recombinant Human FCAR/CD89 Protein |
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Catalog No. | RP00113 |
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Description | Recombinant Human FCAR/CD89 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln22-Asn227) of human CD89/FCAR (Accession #NP_001991.1) fused with an Fc, 6×His tag at the C-terminus. |
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Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Human IGHA1 (Catalog: RPT0023) at 5 μg/mL (100 μL/well) can bind Human CD89/FCAR (Catalog: RP00113) with a linear range of 0.01-7.4 ng/mL.
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Purity | > 97% by SDS-PAGE. |
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Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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Species | Human |
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Calculated MW | 50.27 kDa |
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Background
This protein is a member of the immunoglobulin gene superfamily and receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Product information
Synonym | FCAR; CD89; CTB-61M7.2; FcalphaRI |
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Expression Host | HEK293 cells |
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Tag | C-hFc&His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.