제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human CD89 mAb (A23349)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry:1X10^6 293F cells (negative control, Left) and 293F(Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human CD89 mAb(A23349, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD89 mAb(A23349, 5 μl/Test, right).

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit anti-Human CD89 mAb(A23349, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD89 mAb(A23349, 5 μl/Test, right)

Overview

Product name	ABflo® 647 Rabbit anti-Human CD89 mAb
Catalog No.	A23349
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59839-ABflo647

Background

This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen information

Immunogen	Recombinant protein of human CD89.
Sequence	QEGDFPMPFI SAKSSPVIPL DGSVKIQCQA IREAYLTQLM IIKNSTYREI GRRLKFWNET DPEFVIDHMD ANKAGRYQCQ YRIGHYRFRY SDTLELVVTG LYGKPFLSAD RGLVLMPGEN ISLTCSSAHI PFDRFSLAKE GELSLPQHQS GEHPANFSLG PVDLNVSGIY RCYGWYNRSP YLWSFPSNAL ELVVTDSIHQ DYTTQN
Gene ID
Swiss Prot
Synonyms	CD89; FcalphaR; FcalphaRI; CTB-61M7.2
Calculated MW	32kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Plasma membrane, Secreted.

Documents

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Research Area

ABflo® 647 Rabbit anti-Human CD89 mAb images

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD89 mAb (A23349)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD89 mAb(A23349, 5 μl/Test, right)

* For research use only. Not for therapeutic or diagnostic purposes.