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Recombinant Human Collagen I alpha 1/COL1A1 Protein (RP01842)

Datasheet

Overview

Product nameRecombinant Human Collagen I alpha 1/COL1A1 Protein
Catalog No.RP01842
DescriptionRecombinant Human Collagen I alpha 1/COL1A1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp1219-Leu1464) of human Collagen I alpha 1/COL1A1 (Accession #NP_000079.2) fused with and his tag at the C-terminus.
Endotoxin< 0.1 EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesHuman
Calculated MW28.27 kDa
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides . The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues . Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described . This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.
SynonymOI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; Collagen I alpha 1; COL1A1
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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