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Collagen I/COL1A1 Rabbit pAb (A1352)

Publications (124) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Collagen I/COL1A1 Rabbit pAb (A1352)

Western blot analysis of various lysates using Collagen I/COL1A1 Rabbit pAb (A1352) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HT-29
Exposure time: 90s.

ABclonal:Immunohistochemistry - Collagen I/COL1A1 Rabbit pAb (A1352)

Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using Collagen I/COL1A1 Rabbit pAb (A1352) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)

Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (A1352) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)

Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (A1352) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)

Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (A1352) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCollagen I/COL1A1 Rabbit pAb
Catalog No.A1352
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1151-1250 of human Collagen I/COL1A1 (NP_000079.2).
SequenceGKDGLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKDKRHVWFGESMTDGFQFEYGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIRAEGNSRFTYSVTVDGCTSHTGAWGKTVIEYKTTKTSRLPIIDVAPLDVGAPDQEFGFDVGPVCFL
Gene ID
Swiss Prot
SynonymsOI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; Collagen I/COL1A1
Calculated MW139kDa
Observed MW120-130kDa/220kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat skin, Mouse skin, SK-N-SH
Cellular locationSecreted, extracellular matrix, extracellular space.
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus)

.(Mus musculus)

IHC(Mus musculus, Homo sapiens, Bovine, Rattus norvegicus, Mus musculus)

IF(Homo sapiens, Mus musculus, Mus musculus, Ovis aries, Rattus norvegicus, Other)

IHC(Mus musculus , Rattus norvegicus)

IF(Homo sapiens, Rattus norvegicus, Mus musculus)

WB(Homo sapiens,Mus musculus)

RT-qPCR(Rattus norvegicus)

IHC(Mus musculus)

qPCR(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Collagen I/COL1A1 Rabbit pAb (A1352)}

    Western blot - Collagen I/COL1A1 Rabbit pAb (A1352)

    Western blot analysis of various lysates using Collagen I/COL1A1 Rabbit pAb (A1352) at 1:3000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): HT-29
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - Collagen I/COL1A1 Rabbit pAb (A1352)}

    Immunohistochemistry - Collagen I/COL1A1 Rabbit pAb (A1352)

    Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using Collagen I/COL1A1 Rabbit pAb (A1352) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)}

    Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)

    Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (A1352) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)}

    Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)

    Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (A1352) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)}

    Immunofluorescence - Collagen I/COL1A1 Rabbit pAb (A1352)

    Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit pAb (A1352) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A1352? Please let us know so that we can cite the reference in this datasheet.

    항체 (6)

    Collagen I/COL1A1 Rabbit pAb
    A16891

    WBIHC-PIF/ICCELISA

    Reactivity:Human, Mouse, Rat

    Collagen I/COL1A1 Rabbit pAb

    Collagen I/COL1A1 Rabbit mAb
    A22090

    WBIHC-PIF/ICCELISA

    Reactivity:Human, Mouse, Rat

    Collagen I/COL1A1 Rabbit mAb

    Collagen I/COL1A1 Rabbit mAb
    A24112

    WBIHC-PIF/ICCELISA

    Reactivity:Human, Mouse

    Collagen I/COL1A1 Rabbit mAb

    Human Pro-Collagen I alpha Rabbit pAb
    A24863

    WBIHC-PIF/ICC

    Reactivity:Human

    Human Pro-Collagen I alpha Rabbit pAb

    COL1A1 Rabbit pAb
    A25439

    ELISAWBIF/ICC

    Reactivity:Human, Mouse

    COL1A1 Rabbit pAb

    Pro-Collagen I/COL1A1 Rabbit mAb
    A26946

    WBIHC-PIF/ICCIPELISA

    Reactivity:Human

    Pro-Collagen I/COL1A1 Rabbit mAb

    ELISA 키트 (1)

    Human Collagen Type I Alpha 1 ELISA Kit (COL1a1)
    RK01149

    Human Collagen Type I Alpha 1 ELISA Kit (COL1a1)

    재조합 단백질 (2)

    Recombinant Human Collagen I alpha 1/COL1A1 Protein
    RP01842

    Tags:C-His

    Expressed Host:HEK293 cells

    Recombinant Human Collagen I alpha 1/COL1A1 Protein

    Recombinant Human Collagen I alpha 1/COL1A1 Protein
    RP01886

    Tags:C-hFc

    Expressed Host:HEK293 cells

    Recombinant Human Collagen I alpha 1/COL1A1 Protein

    Secondary Antibodies (26)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
    AS063

    WB

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)