제품 > 항체 > 단일클론항체(mAb)

Collagen I/COL1A1 Rabbit mAb (A22090)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Collagen I/COL1A1 Rabbit mAb (A22090) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Collagen I/COL1A1 Rabbit mAb (A22090)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Collagen I/COL1A1 Rabbit mAb (A22090) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Collagen I/COL1A1 Rabbit mAb (A22090) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Collagen I/COL1A1 Rabbit mAb (A22090) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit mAb (A22090)

Confocal imaging of NIH-3T3 cells using Collagen I/COL1A1 Rabbit mAb (A22090, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product name	Collagen I/COL1A1 Rabbit mAb
Catalog No.	A22090
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53610

Background

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1151-1250 of human Collagen I/COL1A1 (NP_000079.2).
Sequence	GKDGLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEG
Gene ID
Swiss Prot
Synonyms	OI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; Collagen I/COL1A1
Calculated MW	139kDa
Observed MW	220kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:500 - 1:2000 IF/ICC 1:200 - 1:800 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	NIH/3T3
Cellular location	Secreted, extracellular matrix, extracellular space.
Customer validation	WB(Mus musculus) IF(Mus musculus, Homo sapiens) WB(Mus musculus, Homo sapiens) IHC(Mus musculus)

Documents

Certificate of Compliance

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Research Area

Collagen I/COL1A1 Rabbit mAb images

Western blot - Collagen I/COL1A1 Rabbit mAb (A22090)

Immunohistochemistry - Collagen I/COL1A1 Rabbit mAb (A22090)

Immunofluorescence - Collagen I/COL1A1 Rabbit mAb (A22090)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

PeerJ

Application:

IF:

2.3

Species:

Mus musculus

PMID:

37790634

Title:

Loss of Farnesoid X receptor (FXR) accelerates dysregulated glucose and renal injury in db/db mice - PMC

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

Journal of ethnopharmacology

Application:

IF, WB

IF:

4.8

Species:

Mus musculus

PMID:

38171465

Title:

Bletilla striata polysaccharide attenuated the progression of pulmonary fibrosis by inhibiting TGF-1/Smad signaling pathway

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

Bioorganic chemistry

Application:

IF:

4.5

Species:

Mus musculus

PMID:

38537336

Title:

Qingkailing granule alleviates pulmonary fibrosis by inhibiting PI3K/AKT and SRC/STAT3 signaling pathways

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

Journal of controlled release : official journal of the Controlled Release Society

Application:

IF, WB

IF:

10.5

Species:

Homo sapiens

PMID:

38648957

Title:

Cadherin-11 targeted cell-specific liposomes enabled skin fibrosis treatment by inducing apoptosis

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

38926800

Title:

Activation of the Wnt/-catenin signalling pathway enhances exosome production by hucMSCs and improves their capability to promote diabetic wound healing - PMC

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

Journal of stomatology, oral and maxillofacial surgery

Application:

IF:

1.8

Species:

Homo sapiens

PMID:

38815725

Title:

Photothermal Modulation of Gingival Fibroblasts via Polydopamine-Coated Zirconia: A Novel Approach for Promoting Peri-Implant Soft Tissue Integration

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

The Science of the total environment

Application:

IHC, WB

IF:

8.2

Species:

Mus musculus

PMID:

38278228

Title:

Inhaled polystyrene nanoplastics induced liver injury and fibrosis by activating ferroptosis pathways

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

Cell discovery

Application:

IF:

33.5

Species:

Mus musculus

PMID:

39187483

Title:

Gut microbiota-derived indole-3-acetic acid suppresses high myopia progression by promoting type I collagen synthesis - PMC

References for Collagen I/COL1A1 Rabbit mAb (A22090)

Product:

Collagen I/COL1A1 Rabbit mAb

Journal:

Gut microbes

Application:

IF:

12.2

Species:

Mus musculus

PMID:

39410876

Title:

High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis - PMC