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Collagen I/COL1A1 Rabbit mAb (A24112)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Collagen I/COL1A1 Rabbit mAb (A24112)

Western blot analysis of various lysates using Collagen I/COL1A1 Rabbit mAb (A24112) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HT-29.
Exposure time: 90s.

ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit mAb (A24112)

Confocal imaging of U-138 MG cells using Collagen I/COL1A1 Rabbit mAb (A24112, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit mAb (A24112)

Confocal imaging of NIH/3T3 cells using Collagen I/COL1A1 Rabbit mAb (A24112, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunohistochemistry - Collagen I/COL1A1 Rabbit mAb (A24112)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Collagen I/COL1A1 Rabbit mAb (A24112) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Collagen I/COL1A1 Rabbit mAb (A24112)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Collagen I/COL1A1 Rabbit mAb (A24112) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameCollagen I/COL1A1 Rabbit mAb
Catalog No.A24112
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53617
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1150-1250 of human Collagen I/COL1A1 (NP_000079.2).
SequencePGKDGLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEG
Gene ID
Swiss Prot
SynonymsOI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; Collagen I/COL1A1
Calculated MW139kDa
Observed MW130kDa,220kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU118-MG, U251-MG
Cellular locationSecreted, extracellular matrix, extracellular space.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Collagen I/COL1A1 Rabbit mAb (A24112)}

    Western blot - Collagen I/COL1A1 Rabbit mAb (A24112)

    Western blot analysis of various lysates using Collagen I/COL1A1 Rabbit mAb (A24112) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): HT-29.
    Exposure time: 90s.
    ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit mAb (A24112)}

    Immunofluorescence - Collagen I/COL1A1 Rabbit mAb (A24112)

    Confocal imaging of U-138 MG cells using Collagen I/COL1A1 Rabbit mAb (A24112, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - Collagen I/COL1A1 Rabbit mAb (A24112)}

    Immunofluorescence - Collagen I/COL1A1 Rabbit mAb (A24112)

    Confocal imaging of NIH/3T3 cells using Collagen I/COL1A1 Rabbit mAb (A24112, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunohistochemistry - Collagen I/COL1A1 Rabbit mAb (A24112)}

    Immunohistochemistry - Collagen I/COL1A1 Rabbit mAb (A24112)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Collagen I/COL1A1 Rabbit mAb (A24112) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Collagen I/COL1A1 Rabbit mAb (A24112)}

    Immunohistochemistry - Collagen I/COL1A1 Rabbit mAb (A24112)

    Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Collagen I/COL1A1 Rabbit mAb (A24112) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A24112? Please let us know so that we can cite the reference in this datasheet.

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