Recombinant Human IL-2RA/CD25 Protein (RP01405)

Overview

Product name	Recombinant Human IL-2RA/CD25 Protein
Catalog No.	RP01405
Description	Recombinant Human IL-2RA/CD25 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu22-Cys213) of human CD25/IL-2Ralpha (Accession #NP_000408.1) fused with a Fc, 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized human IL2 (Catalog: RP01039) at 2 μg/ml (100 μL/well) can bind human IL2RA with a linear range of 0.01-396.60 ng/mL
Purity	> 95% by SDS-PAGE.
Endotoxin	<0.1EU/μg
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Human
Calculated MW	48.53 kDa

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown.

Product information

Synonym	IL2RA; CD25; IDDM10; IL2R; IMD41; TCGFR; p55; interleukin-2 receptor subunit alpha; CD25; IDDM10; IL2R; IMD41; TCGFR; p55
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.