Biotinylated Recombinant Human IL-2 R alpha/CD25 Protein (RP00449B)

Overview

Product name	Biotinylated Recombinant Human IL-2 R alpha/CD25 Protein
Catalog No.	RP00449B
Description	Biotinylated Recombinant Human IL-2 R alpha/CD25 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu22-Cys213) of Human IL-2 R alpha/CD25 (Accession #P01589) fused with a C-His&Avi tag at the C-terminus.
Purity	> 95% by SDS-PAGE；> 95% by HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species	Human
Calculated MW	24.6 kDa

Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL?2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Product information

Synonym	CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
Expression Host	HEK293 cells
Tag	C-His&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.