제품 > 항체 > 단일클론항체(mAb)

APC Rabbit anti-Human CD25 mAb (A26774)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were intracellularly-stained with APC Rabbit anti-Human CD25 mAb (A26774, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD25 mAb (A26774, 5 μl/Test, right).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD25 mAb (A26774, 5 μl/Test, right).Cells in the Lymphocytes gate were used for analysis.

Overview

Product name	APC Rabbit anti-Human CD25 mAb
Catalog No.	A26774
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC58045-APC

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-213 of human CD25 (NP_000408.1).
Sequence	ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC
Gene ID
Swiss Prot
Synonyms	p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Calculated MW	31kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	external side of plasma membrane, plasma membrane, cell surface, Membrane, Single-pass type I membrane protein, Plasma membrane.

Documents

Certificate of Compliance

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Research Area

APC Rabbit anti-Human CD25 mAb images

Flow CytoMetry - APC Rabbit anti-Human CD25 mAb (A26774)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD25 mAb (A26774, 5 μl/Test, right).

Flow CytoMetry - APC Rabbit anti-Human CD25 mAb (A26774)

* For research use only. Not for therapeutic or diagnostic purposes.