Overview
| Product name | Recombinant Mouse Fc gamma RI/FCGR1/CD64 Protein |
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| Catalog No. | RP00710 |
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| Description | Recombinant Mouse Fc gamma RI/FCGR1/CD64 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Glu25-Pro297) of mouse Fc γ RI/FCGR1A/CD64 (Accession #P26151) fused with a 6×His tag at the C-terminus. |
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation. |
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| Species | Mouse |
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| Calculated MW | 31.5 kDa |
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Background
CD64, also known as Fc-gamma receptor 1 (Fc γ RI), is a type of integral membrane glycoprotein that bindsmonomeric IgG-type antibodies with high affinity. After binding IgG, CD64 interacts with an accessory chainknown as the common γ chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellularactivation. CD64 is composed of a signal peptide, three extracellular immunoglobulin domains of the C2-typeused to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 mediatesendocytosis, phagocytosis, antibody-dependent cellular cytotoxicity, cytokine release, and superoxideproduction. It is normally expressed on the surfaces of monocytes and macrophages.
Product information
| Synonym | High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; CD64; Fcgr1 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
