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ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb (A23855)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb (A23855)

Flow cytometry: 1X10^6 BALB/C mouse splenocytes (negative control, left) and RAW 264.7 cells(right) were surface-stained with ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb(A23855, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb (A23855)

Flow cytometry:1X10^6 RAW 264.7 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb(A23855, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb
Catalog No.A23855
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61141-ABflo488
Enables IgG binding activity and IgG receptor activity. Acts upstream of or within several processes, including antibody-dependent cellular cytotoxicity; endocytosis; and positive regulation of hypersensitivity. Located in external side of plasma membrane. Is expressed in liver. Orthologous to several human genes including FCGR1A (Fc gamma receptor Ia).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-297 of mouse FcγRI/CD64 (NP_034316.1).
SequenceEVVNATKAVITLQPPWVSIFQKENVTLWCEGPHLPGDSSTQWFINGTAVQISTPSYSIPEASFQDSGEYRCQIGSSMPSDPVQLQIHNDWLLLQASRRVLTEGEPLALRCHGWKNKLVYNVVFYRNGKSFQFSSDSEVAILKTNLSHSGIYHCSGTGRHRYTSAGVSITVKELFTTPVLRASVSSPFPEGSLVTLNCETNLLLQRPGLQLHFSFYVGSKILEYRNTSSEYHIARAEREDAGFYWCEVATEDSSVLKRSPELELQVLGPQSSAP
Gene ID
Swiss Prot
SynonymsCD64; IGGHAFC; FcgammaRI
Calculated MW44kDa
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb (A23855)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb (A23855)

    Flow cytometry: 1X10^6 BALB/C mouse splenocytes (negative control, left) and RAW 264.7 cells(right) were surface-stained with ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb(A23855, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb (A23855)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb (A23855)

    Flow cytometry:1X10^6 RAW 264.7 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse FcγRI/CD64 mAb(A23855, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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