Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of various lysates using FcγRI/CD64 Rabbit mAb (A24082) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Flow cytometry: 1X10^6 BALB/C mouse splenocyte (negative control, left) and RAW 264.7 cells (right) were surface-stained with FcγRI/CD64 Rabbit mAb (A24082, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
| Product name | FcγRI/CD64 Rabbit mAb |
|---|---|
| Catalog No. | A24082 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC61141 |
| Immunogen | Recombinant Protein corresponding to a sequence within amino acids 25-297 of mouse FcγRI/CD64 (NP_034316.1). |
|---|---|
| Sequence | EVVNATKAVITLQPPWVSIFQKENVTLWCEGPHLPGDSSTQWFINGTAVQISTPSYSIPEASFQDSGEYRCQIGSSMPSDPVQLQIHNDWLLLQASRRVLTEGEPLALRCHGWKNKLVYNVVFYRNGKSFQFSSDSEVAILKTNLSHSGIYHCSGTGRHRYTSAGVSITVKELFTTPVLRASVSSPFPEGSLVTLNCETNLLLQRPGLQLHFSFYVGSKILEYRNTSSEYHIARAEREDAGFYWCEVATEDSSVLKRSPELELQVLGPQSSAP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CD64; IGGHAFC; FcgammaRI; FcγRI/CD64 |
| Calculated MW | 44kDa |
| Observed MW | 45kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Flow Cytometry |
| Positive samples | THP-1, RAW 264.7, Mouse spleen |
| Cellular location | Cell membrane |
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