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MAP1LC3B Rabbit pAb (A21800)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - MAP1LC3B Rabbit pAb (A21800)

Western blot analysis of lysates from 293T cells using MAP1LC3B Rabbit pAb (A21800) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - MAP1LC3B Rabbit pAb (A21800)

Western blot analysis of lysates from C6 cells using MAP1LC3B Rabbit pAb (A21800) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameMAP1LC3B Rabbit pAb
Catalog No.A21800
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-50 of human MAP1LC3B (NP_073729.1).
SequenceMPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKT
Gene ID
Swiss Prot
SynonymsLC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; 3B
Calculated MW14kDa
Observed MW14kDa, 16kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by Chloroquine, C6 treated by Chloroquine
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton.

Documents

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ABclonal:Western blot - MAP1LC3B Rabbit pAb (A21800)}

Western blot - MAP1LC3B Rabbit pAb (A21800)

Western blot analysis of lysates from 293T cells using MAP1LC3B Rabbit pAb (A21800) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - MAP1LC3B Rabbit pAb (A21800)}

Western blot - MAP1LC3B Rabbit pAb (A21800)

Western blot analysis of lysates from C6 cells using MAP1LC3B Rabbit pAb (A21800) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (4)

ELISA 키트 (1)

Secondary Antibodies (26)