제품 > 항체 > 다중클론항체(pAb)

[KD Validated] LC3B Rabbit pAb (A7198)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] LC3B Rabbit pAb (A7198)

Western blot analysis of various lysates using [KD Validated] LC3B Rabbit pAb (A7198) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from wild type (WT) and LC3B knockdown (KD) 293T, 293T+chloroquine cells, using [KD Validated] LC3B Rabbit pAb (A7198) at 1:1000 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KD Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry analysis of paraffin-embedded Rat brain using [KD Validated] LC3B Rabbit pAb (A7198) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using [KD Validated] LC3B Rabbit pAb (A7198) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human stomach using [KD Validated] LC3B Rabbit pAb (A7198) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KD Validated] LC3B Rabbit pAb (A7198)

Immunofluorescence analysis of paraffin-embedded rat brain using [KD Validated] LC3B Rabbit pAb (A7198) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	[KD Validated] LC3B Rabbit pAb
Catalog No.	A7198
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1).
Sequence	MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYE
Gene ID
Swiss Prot
Synonyms	LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; 3B
Calculated MW	15kDa
Observed MW	14kDa/16kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293T treated by Chloroquine, NIH/3T3 treated by Chloroquine, C6 treated by Chloroquine
Cellular location	Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer validation	(MSC cells, Human lung cancer cell, C2C12, Squamous cell carcinoma, Mouse cells, 293T, U87MG, Rat cells) WB(Mus musculus, Sus scrofa, Homo sapiens, Rattus norvegicus, Danio rerio, Homo sapiens, Bombyx mori Linnaeus) IF(Mus musculus, Homo sapiens) IHC(Homo sapiens, Mus musculus) IF(Homo sapiens, Rattus norvegicus) IHC(Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] LC3B Rabbit pAb images

Western blot - [KD Validated] LC3B Rabbit pAb (A7198)

Immunohistochemistry - [KD Validated] LC3B Rabbit pAb (A7198)

Immunofluorescence - [KD Validated] LC3B Rabbit pAb (A7198)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A7198

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

European Journal of Pharmacology

Application:

IF:

4.432

Species:

Mus musculus

PMID:

27887947

Title:

Berberine enhances the AMPK activation and autophagy and mitigates high glucose-induced apoptosis of mouse podocytes

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Viruses

Application:

IF:

5.04

Species:

Sus scrofa

PMID:

31109068

Title:

Characteristics of the Life Cycle of Porcine Deltacoronavirus (PDCoV) In Vitro: Replication Kinetics, Cellular Ultrastructure and Virion Morphology, and Evidence of Inducing Autophagy.

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Theriogenology

Application:

IF:

2.74

Species:

Sus scrofa

PMID:

31437668

Title:

Lysosomal dysfunction disturbs porcine oocyte maturation and developmental capacity by disorganizing chromosome/cytoskeleton and activating autophagy/apoptosis.

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

International Immunopharmacology

Application:

IF:

4.932

Species:

Mus musculus

PMID:

31655340

Title:

Macrophages-derived p38α promotes the experimental severe acute pancreatitis by regulating inflammation and autophagy

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Journal of Cellular Biochemistry

Application:

IF:

4.429

Species:

Homo sapiens

PMID:

31904151

Title:

miR-133a-3p/FOXP3 axis regulates cell proliferation and autophagy in gastric cancer

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

European Journal of Pharmacology

Application:

IF:

4.432

Species:

Mus musculus

PMID:

32001218

Title:

Highly bioavailable berberine formulation ameliorates diabetic nephropathy through the inhibition of glomerular mesangial matrix expansion and the activation of autophagy

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Journal of Agricultural and Food Chemistry

Application:

IF:

5.279

Species:

Rattus norvegicus

PMID:

32090563

Title:

Neuroprotection by Walnut-Derived Peptides through Autophagy Promotion via Akt/mTOR Signaling Pathway against Oxidative Stress in PC12 Cells

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.81

Species:

Rattus norvegicus

PMID:

32161543

Title:

Ferulic Acid Attenuates Hypoxia/Reoxygenation Injury by Suppressing Mitophagy Through the PINK1/Parkin Signaling Pathway in H9c2 Cells

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

International journal of biological sciences

Application:

IF:

6.58

Species:

Mus musculus

PMID:

32398956

Title:

Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Cardiovascular Diagnosis and Therapy

Application:

IF:

2.845

Species:

Mus musculus

PMID:

32420095

Title:

Ginsenoside Rg3 protects heart against isoproterenol-induced myocardial infarction by activating AMPK mediated autophagy.

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Journal of Neuroinflammation

Application:

IF:

8.322

Species:

Mus musculus

PMID:

33158440

Title:

Deficiency of microglial Hv1 channel is associated with activation of autophagic pathway and ROS production in LPC-induced demyelination mouse model

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

PLoS Pathog

Application:

IF:

6.823

Species:

Homo sapiens

PMID:

33539420

Title:

CircRNA ARFGEF1 functions as a ceRNA to promote oncogenic KSHV-encoded viral interferon regulatory factor induction of cell invasion and angiogenesis by upregulating glutaredoxin 3

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

J Photochem Photobiol B

Application:

IF:

6.252

Species:

Mus musculus

PMID:

33316624

Title:

The role of FOS-mediated autophagy activation in the indocyanine green-based photodynamic therapy for treating melanoma

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Life Sci

Application:

IF:

5.037

Species:

Mus musculus

PMID:

33617857

Title:

Identification of GL-V9 as a novel senolytic agent against senescent breast cancer cells

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Exp Eye Res

Application:

IF:

3.467

Species:

Rattus norvegicus

PMID:

33636209

Title:

Melatonin exerts protective effects on diabetic retinopathy via inhibition of Wnt/β-catenin pathway as revealed by quantitative proteomics

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Autophagy

Application:

IF:

16.01

Species:

Homo sapiens

PMID:

33719859

Title:

Selective autophagy receptor SQSTM1/ p62 inhibits Seneca Valley virus replication by targeting viral VP1 and VP3

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Food Nutr Res

Application:

IF:

3.89

Species:

Mus musculus

PMID:

33841067

Title:

Fisetin inhibits inflammation and induces autophagy by mediating PI3K/AKT/mTOR signaling in LPS-induced RAW264.7 cells

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Biochem Biophys Res Commun

Application:

IF:

3.57

Species:

Homo sapiens

PMID:

33784507

Title:

Nuclear receptor coactivator 4-mediated ferritinophagy drives proliferation of dental pulp stem cells in hypoxia

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Vet Microbiol

Application:

IF:

3.29

Species:

Mus musculus

PMID:

33991788

Title:

Autophagy is a defense mechanism controlling Streptococcus suis serotype 2 infection in murine microglia cells

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Theriogenology

Application:

IF:

2.74

Species:

Sus scrofa

PMID:

34653791

Title:

Melatonin mitigates Chloroquine-induced defects in porcine immature Sertoli cells

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Breast cancer (Tokyo, Japan)

Application:

IF:

4.23

Species:

Homo sapiens

PMID:

32654094

Title:

In vitro anti-breast cancer studies of LED red light therapy through autophagy

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

4.379

Species:

Danio rerio

PMID:

31827208

Title:

Fundc1 is necessary for proper body axis formation during embryogenesis in zebrafish

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Autophagy

Application:

IF:

13.39

Species:

Homo sapiens

PMID:

36037300

Title:

Genome-Scale CRISPR Screen Identifies LAPTM5 driving lenvatinib resistance in hepatocellular carcinoma

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Environmental toxicology

Application:

IF:

4.1

Species:

Rattus norvegicus

PMID:

34608745

Title:

LncRNA MEG3 restrained pulmonary fibrosis induced by NiO NPs via regulating hedgehog signaling pathway‐mediated autophagy

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Mus musculus

PMID:

37027942

Title:

SIRT1/FOXO3-mediated autophagy signaling involved in manganese-induced neuroinflammation in microglia

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Open medicine (Warsaw, Poland)

Application:

IF:

Species:

Homo sapiens

PMID:

36941991

Title:

Exosomal miR-93-5p regulated the progression of osteoarthritis by targeting ADAMTS9 - PMC

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Journal of biomedical science

Application:

IF:

12.77

Species:

Homo sapiens

PMID:

37537557

Title:

Optimized allotopic expression of mitochondrial ND6 transgene restored complex I and apoptosis deficiencies caused by LHON-linked ND6 14484T>C mutation - PMC

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

Species:

Rattus norvegicus, Homo sapiens

PMID:

37932279

Title:

ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis - PMC

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

The EMBO Journal

Application:

WB, IHC, IF

IF:

14.012

Species:

Homo sapiens

PMID:

32368828

Title:

RNA m6 A methylation regulates sorafenib resistance in liver cancer through FOXO3-mediated autophagy

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Food and chemical toxicology

Application:

IF:

5.572

Species:

Homo sapiens

PMID:

30578841

Title:

Corynoxine B ameliorates HMGB1-dependent autophagy dysfunction during manganese exposure in SH-SY5Y human neuroblastoma cells.

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Am J Cancer Res

Application:

IF:

5.942

Species:

Homo sapiens

PMID:

33294275

Title:

MOB1A regulates glucose deprivation-induced autophagy via IL6-STAT3 pathway in gallbladder carcinoma

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Molecular therapy : the journal of the American Society of Gene Therapy

Application:

WB, IHC, IF

IF:

12.91

Species:

Homo sapiens

PMID:

36307991

Title:

N6-methyladenosine demethylase FTO enhances chemo-resistance in colorectal cancer through SIVA1-mediated apoptosis

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Analytical cellular pathology (Amsterdam)

Application:

IF:

4.13

Species:

Homo sapiens

PMID:

36247874

Title:

Chemoprevention of 4NQO-Induced Mouse Tongue Carcinogenesis by AKT Inhibitor through the MMP-9/RhoC Signaling Pathway and Autophagy - PMC

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Analytical cellular pathology (Amsterdam)

Application:

IHC, IF

IF:

4.13

Species:

Homo sapiens

PMID:

36247874

Title:

Chemoprevention of 4NQO-Induced Mouse Tongue Carcinogenesis by AKT Inhibitor through the MMP-9/RhoC Signaling Pathway and Autophagy - PMC

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Gene

Application:

IF:

3.91

Species:

Mus musculus

PMID:

38185293

Title:

Running Improves Muscle Mass by Activating Autophagic Flux and Inhibiting Ubiquitination Degradation in Mdx Mice

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Life Sci

Application:

IF:

6.78

Species:

Rattus norvegicus

PMID:

33774032

Title:

Astragaloside IV-targeting miRNA-1 attenuates lipopolysaccharide-induced cardiac dysfunction in rats through inhibition of apoptosis and autophagy

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Life Sci

Application:

IF:

6.78

Species:

Rattus norvegicus

PMID:

33774032

Title:

Astragaloside IV-targeting miRNA-1 attenuates lipopolysaccharide-induced cardiac dysfunction in rats through inhibition of apoptosis and autophagy

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Cell death and differentiation

Application:

WB, IHC

IF:

12.067

Species:

Mus musculus

PMID:

30683918

Title:

The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling.

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Neurochemical research

Application:

IHC, IF

IF:

4.41

Species:

Rattus norvegicus

PMID:

33201400

Title:

Protective effects of curcumin against paclitaxel-induced spinal cord and sciatic nerve injuries in rats

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Journal of pharmaceutical analysis

Application:

IF:

8.8

Species:

Homo sapiens

PMID:

39021382

Title:

In situ visualization of the cellular uptake and sub-cellular distribution of mussel oligosaccharides

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Homo sapiens

PMID:

39229529

Title:

BRF1 promotes the odontogenic differentiation of dental pulp stem cells in pulpitis by inducing autophagy - PMC

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Heliyon

Application:

IHC

IF:

Species:

Homo sapiens

PMID:

39229529

Title:

BRF1 promotes the odontogenic differentiation of dental pulp stem cells in pulpitis by inducing autophagy - PMC

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Insect Biochemistry and Molecular Biology

Application:

IF:

3.8

Species:

Bombyx mori Linnaeus

PMID:

31654713

Title:

Steroid hormone 20-hydroxyecdysone induces the transcription and complex assembly of V-ATPases to facilitate autophagy in Bombyx mori

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Current issues in molecular biology

Application:

IF:

3.1

Species:

Homo sapiens

PMID:

39329902

Title:

Cacalol Acetate as Anticancer Agent: Antiproliferative, Pro-Apoptotic, Cytostatic, and Anti-Migratory Effects - PMC

References for [KD Validated] LC3B Rabbit pAb (A7198)

Product:

[KD Validated] LC3B Rabbit pAb

Journal:

Journal of virology

Application:

IF:

5.4

Species:

Homo sapiens

PMID:

38189249

Title:

N-terminal acetyltransferase 6 facilitates enterovirus 71 replication by regulating PI4KB expression and replication organelle biogenesis - PMC

Publishing research using A7198? Please let us know so that we can cite the reference in this datasheet.

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제품 > 항체 > 다중클론항체(pAb)

[KD Validated] LC3B Rabbit pAb (A7198)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

[KD Validated] LC3B Rabbit pAb images

Submit an review for A7198

Reviews List

Product Protocols

항체 (4)

ELISA 키트 (1)

Secondary Antibodies (26)