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[KD Validated] LC3B Rabbit mAb (A19665)

Reviews (3)Publications (154) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of various lysates, using [KD Validated] LC3B Rabbit mAb (A19665) at 1:1000 dilution. 293T, C6 and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KD Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of lysates from wild type(WT) and LC3B knockdown (KD) 293T cells, using [KD Validated] LC3B Rabbit mAb (A19665) at 1:1000 dilution. wild type(WT) and LC3B knockdown (KD) 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KD Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry analysis of paraffin-embedded Human brain using [KD Validated] LC3B Rabbit mAb (A19665) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry analysis of paraffin-embedded Rat brain using [KD Validated] LC3B Rabbit mAb (A19665) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)

Confocal imaging of HeLa cells (treated with Chloroquine) and HeLa cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)

Confocal imaging of C6 cells (treated with Chloroquine) and C6 cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)

Confocal imaging of NIH/3T3 cells (treated with Chloroquine) and NIH/3T3 cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KD Validated] LC3B Rabbit mAb (A19665)

Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KD Validated] LC3B Rabbit mAb (A19665). Western blot was performed from the immunoprecipitate using LC3B antibody (A19665) at a dilution of 1:1000.

Review
ABclonal: review for [KD Validated] LC3B Rabbit mAb(A19665)
Review
ABclonal: review for [KD Validated] LC3B Rabbit mAb(A19665)
Review
ABclonal: review for [KD Validated] LC3B Rabbit mAb(A19665)

Overview

Product name[KD Validated] LC3B Rabbit mAb
Catalog No.A19665
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0144
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B (Q9GZQ8).
SequencePSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDG
Gene ID
Swiss Prot
SynonymsLC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; 3B
Calculated MW15kDa
Observed MW14kDa/16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:200 - 1:800
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T treated by Chloroquine, C6 treated by Chloroquine, NIH/3T3 treated by Chloroquine
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton.
Customer validation

(SHY5Y cells, Hela)

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Gallus gallus, Sus scrofa, Ctenopharyngodon idellus, Dianella ensifolia, Mus musculus, Other, Homo sapiens, Capra hircus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus, Other, Oryctolagus cuniculus)

IHC(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Mus musculus, Capra hircus)

IF(Homo sapiens, Mus musculus, Gallus gallus, Mus musculus, Homo sapiens)

WB(Homo sapiens, Mus musculus, Oryctolagus cuniculus)

ELISA(Mus musculus)

IHC(Homo sapiens,Rattus norvegicus, Mus musculus)

WB(Homo sapiens)

Cell-based assay(Homo sapiens)

Other(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KD Validated] LC3B Rabbit mAb (A19665)}

Western blot - [KD Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of various lysates, using [KD Validated] LC3B Rabbit mAb (A19665) at 1:1000 dilution. 293T, C6 and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KD Validated] LC3B Rabbit mAb (A19665)}

Western blot - [KD Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of lysates from wild type(WT) and LC3B knockdown (KD) 293T cells, using [KD Validated] LC3B Rabbit mAb (A19665) at 1:1000 dilution. wild type(WT) and LC3B knockdown (KD) 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KD Validated] LC3B Rabbit mAb (A19665)}

Immunohistochemistry - [KD Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry analysis of paraffin-embedded Human brain using [KD Validated] LC3B Rabbit mAb (A19665) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] LC3B Rabbit mAb (A19665)}

Immunohistochemistry - [KD Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry analysis of paraffin-embedded Rat brain using [KD Validated] LC3B Rabbit mAb (A19665) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)}

Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)

Confocal imaging of HeLa cells (treated with Chloroquine) and HeLa cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)}

Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)

Confocal imaging of C6 cells (treated with Chloroquine) and C6 cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)}

Immunofluorescence - [KD Validated] LC3B Rabbit mAb (A19665)

Confocal imaging of NIH/3T3 cells (treated with Chloroquine) and NIH/3T3 cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunoprecipitation - [KD Validated] LC3B Rabbit mAb (A19665)}

Immunoprecipitation - [KD Validated] LC3B Rabbit mAb (A19665)

Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KD Validated] LC3B Rabbit mAb (A19665). Western blot was performed from the immunoprecipitate using LC3B antibody (A19665) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (4)

ELISA 키트 (1)

Secondary Antibodies (26)