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Human CD3 Monoclonal Antibody (YR0010)

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human CD3 (Research Grade Teplizumab Biosimilar) Monoclonal Antibody to CD3. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A donkey anti-human IgG HRP conjugate was used for detection.

ABclonal:Flow CytoMetry - Human CD3 Monoclonal Antibody (YR0010)

Detection of CD3 in Human PBMCs cell line by Flow Cytometry. Human PBMCs cell line was stained with Biotinylated Human Anti-Human CD3(Teplizumab Biosimilar) Monoclonal Antibody followed by APC conjugated NeutrAvidin Secondary Antibody, or unstained cells (open blue histogram).

Basic Information

Clone	Teplizumab Biosimilar
Molecular Weight	150 kDa
Endotoxin	<1EU/mg (<0.001EU/μg)Determined by LAL gel clotting assay
Sterility	0.2 μm filtration
Aggregation	<5% Determined by SECP
Purity	>95% Determined by SDS-PAGE

Product Information

Production	Purified from cell culture supernatant in an animal-free facility
Purification	Protein A or G purification
Storage	2 - 8°C for up to 4 weeks and -80°C for long term storage (Avoid repeated freezing and thawing)

Target Background

Teplizumab is a biosimilar directed against the extracellular domain of CD3. This antibody can be used in flow cytometry or various assay format to measure CD3 expression.