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APC Rabbit-anti Human CD3 mAb (A27111)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - APC Rabbit-anti Human CD3 mAb (A27111)

Flow cytometry: 1X10^6 Raji cells (negative control, left) and Human PBMC (right) were surface-stained with APC Rabbit-anti Human CD3 mAb (A27111, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line). Cells in the lymphocyte gate were used for analysis.

ABclonal:Flow CytoMetry - APC Rabbit-anti Human CD3 mAb (A27111)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit-anti Human CD3 mAb (A27111, 5 μl/Test, right). Cells in the lymphocyte gate were used for analysis.

Overview

Product nameAPC Rabbit-anti Human CD3 mAb
Catalog No.A27111
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC68274-APC
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human CD3 (NP_000724.1).
SequenceMQSGTHWRVLGLCLLSVGVWGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMD
Gene ID
Swiss Prot
SynonymsT3E; TCRE; IMD18; CD3epsilon
Calculated MW23kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein.

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ABclonal:Flow CytoMetry - APC Rabbit-anti Human CD3 mAb (A27111)}

Flow CytoMetry - APC Rabbit-anti Human CD3 mAb (A27111)

Flow cytometry: 1X10^6 Raji cells (negative control, left) and Human PBMC (right) were surface-stained with APC Rabbit-anti Human CD3 mAb (A27111, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line). Cells in the lymphocyte gate were used for analysis.
ABclonal:Flow CytoMetry - APC Rabbit-anti Human CD3 mAb (A27111)}

Flow CytoMetry - APC Rabbit-anti Human CD3 mAb (A27111)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit-anti Human CD3 mAb (A27111, 5 μl/Test, right). Cells in the lymphocyte gate were used for analysis.

* For research use only. Not for therapeutic or diagnostic purposes.

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