CD3E Rabbit mAb (A19017)

The multiplex IHC analysis on paraffin-embedded Human stomach cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD35/CR1 Rabbit mAb (A3661, 1:100) with TSA-TYR-520 (Green), CD3E Rabbit mAb (A19017, 1:2000) with TSA-TYR-570 (Red), and Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-690 (cyan). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.

The multiplex IHC analysis on paraffin-embedded Human T-cell lymphoma tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD35/CR1 Rabbit mAb (A3661, 1:100) with TSA-TYR-520 (Green), CD3E Rabbit mAb (A19017, 1:2000) with TSA-TYR-570 (Red), and Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-690 (cyan). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.

The multiplex IHC analysis on paraffin-embedded Human stomach cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD35/CR1 Rabbit mAb (A3661, 1:100) with TSA-TYR-520 (Green), CD3E Rabbit mAb (A19017, 1:2000) with TSA-TYR-570 (Red), and Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-690 (cyan). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.

The multiplex IHC analysis on paraffin-embedded Human T-cell lymphoma tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD35/CR1 Rabbit mAb (A3661, 1:100) with TSA-TYR-520 (Green), CD3E Rabbit mAb (A19017, 1:2000) with TSA-TYR-570 (Red), and Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-690 (cyan). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.

Western blot analysis of various lysates using CD3E Rabbit mAb (A19017) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): PC-3
Exposure time: 5s.

Western blot analysis of lysates from Mouse spleen using CD3E Rabbit mAb (A19017) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD3E Rabbit mAb (A19017) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using CD3E Rabbit mAb (A19017) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using CD3E Rabbit mAb (A19017) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using CD3E Rabbit mAb (A19017) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD3E Rabbit mAb (A19017) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Confocal imaging of Jurkat cells using CD3E Rabbit mAb (A19017, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of paraffin-embedded Mouse thymus tissue using CD3E Rabbit mAb (A19017, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Rat thymus tissue using CD3E Rabbit mAb (A19017, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.