Biotinylated Recombinant Human CD3 epsilon (23-48) Protein (RP00600B)

Overview

Product name	Biotinylated Recombinant Human CD3 epsilon (23-48) Protein
Catalog No.	RP00600B
Description	Biotinylated Recombinant Human CD3 epsilon (23-48) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp23-Thr48) of Human CD3 epsilon (23-48) (Accession #P07766) fused with a C-hFc&Avi tag at the C-terminus.
Purity	> 95% by SDS-PAGE；> 95% by HPLC
Endotoxin	< 1 EU/μg of the protein by LAL method
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species	Human
Calculated MW	31.3 kDa

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Product information

Synonym	CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Expression Host	HEK293 cells
Tag	C-hFc&Avi
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.