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CD3 Rabbit mAb (A26443)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CD3 Rabbit mAb (A26443)

Western blot analysis of various lysates using CD3 Rabbit mAb (A26443)at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): PC-3
Exposure time: 10s.

ABclonal:Immunohistochemistry - CD3 Rabbit mAb (A26443)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD3 Rabbit mAb (A26443) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD3 Rabbit mAb (A26443)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD3 Rabbit mAb (A26443) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD3 Rabbit mAb (A26443)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CD3 Rabbit mAb (A26443) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD3 Rabbit mAb (A26443)

Confocal imaging of paraffin-embedded Human spleen tissue using CD3 Rabbit mAb (A26443, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - CD3 Rabbit mAb (A26443)

Confocal imaging of paraffin-embedded Human tonsil tissue using CD3 Rabbit mAb (A26443, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Flow CytoMetry - CD3 Rabbit mAb (A26443)

Flow cytometry: 1X10^6 Raji cells (negative control, left) and Human PBMC (right) were surface-stained with CD3 Rabbit mAb (A26443, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - CD3 Rabbit mAb (A26443)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or CD3 Rabbit mAb (A26443, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

Overview

Product nameCD3 Rabbit mAb
Catalog No.A26443
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC68274
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human CD3 (NP_000724.1).
SequenceMQSGTHWRVLGLCLLSVGVWGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMD
Gene ID
Swiss Prot
SynonymsT3E; TCRE; IMD18; CD3epsilon
Calculated MW23kDa
Observed MW23kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • FC 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Flow Cytometry    
Positive samplesMOLT-4
Cellular locationCell membrane, Single-pass type I membrane protein.
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CD3 Rabbit mAb (A26443)}

Western blot - CD3 Rabbit mAb (A26443)

Western blot analysis of various lysates using CD3 Rabbit mAb (A26443)at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): PC-3
Exposure time: 10s.
ABclonal:Immunohistochemistry - CD3 Rabbit mAb (A26443)}

Immunohistochemistry - CD3 Rabbit mAb (A26443)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD3 Rabbit mAb (A26443) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD3 Rabbit mAb (A26443)}

Immunohistochemistry - CD3 Rabbit mAb (A26443)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD3 Rabbit mAb (A26443) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD3 Rabbit mAb (A26443)}

Immunohistochemistry - CD3 Rabbit mAb (A26443)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CD3 Rabbit mAb (A26443) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - CD3 Rabbit mAb (A26443)}

Immunofluorescence - CD3 Rabbit mAb (A26443)

Confocal imaging of paraffin-embedded Human spleen tissue using CD3 Rabbit mAb (A26443, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - CD3 Rabbit mAb (A26443)}

Immunofluorescence - CD3 Rabbit mAb (A26443)

Confocal imaging of paraffin-embedded Human tonsil tissue using CD3 Rabbit mAb (A26443, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Flow CytoMetry - CD3 Rabbit mAb (A26443)}

Flow CytoMetry - CD3 Rabbit mAb (A26443)

Flow cytometry: 1X10^6 Raji cells (negative control, left) and Human PBMC (right) were surface-stained with CD3 Rabbit mAb (A26443, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - CD3 Rabbit mAb (A26443)}

Flow CytoMetry - CD3 Rabbit mAb (A26443)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or CD3 Rabbit mAb (A26443, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A26443? Please let us know so that we can cite the reference in this datasheet.

항체 (18)

재조합 단백질 (5)

Secondary Antibodies (26)