Overview
| Product name | Biotinylated Recombinant Human VEGF-A/VEGF165 Protein |
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| Catalog No. | RP02097B |
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| Description | Biotinylated Recombinant Human VEGF-A/VEGF165 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ala27-Arg191) of Human VEGF165 fused with a His tag and Avi tag at the C-terminal. . |
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| Purity | > 95% by Tris-Bis PAGE |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 22.2 kDa |
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Background
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Product information
| Synonym | VEGFA; MVCD1; VEGF; VPF; vascular endothelial growth factor A; MVCD1; VEGF; VPF; L VEGFA; VEGF A |
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| Expression Host | HEK293 cells |
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| Tag | C-His&Avi |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
