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Recombinant Human Catenin beta-1/CTNNB1 Protein (RP01241)

Bioactivityvalidated

Publication (1) Datasheet

Overview

Product nameRecombinant Human Catenin beta-1/CTNNB1 Protein
Catalog No.RP01241
DescriptionRecombinant Human Catenin beta-1/CTNNB1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Leu781) of human Beta-catenin (Accession #XP_016861227.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CD31 at 1 μg/mL (100 μL/well) can bind Human CTNNB1 with a linear range of 0.3-4.9 μg/ml.
Purity> 90% by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW86.33 kDa
SynonymCTNNB; MRD19; armadillo; CTNNB1; MRD19; armadillo; beta Catenin; CTNNB; catenin beta-1
Expression HostE. coli
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RP01241? Please let us know so that we can cite the reference in this datasheet.

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