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Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-β-Catenin-S552 Rabbit pAb (AP0579) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Western blot analysis of lysates from C2C12 cells, using Phospho-β-Catenin-S552 pAb (AP0579) at 1:1000 dilution or β-Catenin antibody (A11932). C2C12 cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product name	Phospho-β-Catenin-S552 Rabbit pAb
Catalog No.	AP0579
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S552 of human beta Catenin (NP_001091679).
Sequence	RTSMG
Gene ID
Swiss Prot
Synonyms	EVR7; CTNNB; MRD19; NEDSDV; armadillo; Phospho-β-Catenin-S552
Calculated MW	85kDa
Observed MW	106kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	C2C12, NIH/3T3, NIH/3T3 treated by Forskolin
Cellular location	Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Customer validation	WB(Homo sapiens, Rattus norvegicus) IHC(Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

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Research Area

Phospho-β-Catenin-S552 Rabbit pAb images

Western blot - Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Product:

Phospho-β-Catenin-S552 Rabbit pAb

Journal:

Oncotarget

Application:

IF:

3.765

Species:

Homo sapiens

PMID:

27835587

Title:

Nerve growth factor modulates the tumor cells migration in ovarian cancer through the WNT/β-catenin pathway.

References for Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Product:

Phospho-β-Catenin-S552 Rabbit pAb

Journal:

Journal Of Molecular Endocrinology

Application:

IF:

5.098

Species:

Rattus norvegicus

PMID:

26386043

Title:

GLP1 protects cardiomyocytes from palmitate-induced apoptosis via Akt/GSK3b/b-catenin pathway

References for Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Product:

Phospho-β-Catenin-S552 Rabbit pAb

Journal:

Stem Cell Res Ther

Application:

IF:

6.832

Species:

Rattus norvegicus

PMID:

33712069

Title:

Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells by canonical Wnt/β-catenin pathway and prevents bone loss in an ovariectomized rat model.

References for Phospho-β-Catenin-S552 Rabbit pAb (AP0579)

Product:

Phospho-β-Catenin-S552 Rabbit pAb

Journal:

Cells

Application:

IF:

7.66

Species:

Homo sapiens

PMID:

36231050