제품 > 항체 > 다중클론항체(pAb)

β-Catenin Rabbit pAb (A11512)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using β-Catenin Rabbit pAb (A11512) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - β-Catenin Rabbit pAb (A11512)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using β-Catenin Rabbit pAb (A11512) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunoprecipitation - β-Catenin Rabbit pAb (A11512)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg β-Catenin antibody (A11512). Western blot was performed from the immunoprecipitate using β-Catenin antibody (A11512) at a dilution of 1:1000.

Review

Overview

Product name	β-Catenin Rabbit pAb
Catalog No.	A11512
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 667-781 of human β-Catenin (NP_001895.1).
Sequence	PQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL
Gene ID
Swiss Prot
Synonyms	EVR7; CTNNB; MRD19; NEDSDV; armadillo; β-Catenin
Calculated MW	85kDa
Observed MW	92kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IHC-P 1:20 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	Mouse brain, Mouse lung, C6
Cellular location	Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Customer validation	(Adenocarcinoma human alveolar basal epithelial cells, rat cells) WB(Mus musculus, Danio rerio, Homo sapiens, Rattus norvegicus, Capra hircus, Mus musculus, Gallus gallus) IHC(Homo sapiens,Rattus norvegicus) IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

β-Catenin Rabbit pAb images

Western blot - β-Catenin Rabbit pAb (A11512)

Immunohistochemistry - β-Catenin Rabbit pAb (A11512)

Immunoprecipitation - β-Catenin Rabbit pAb (A11512)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Pharmacological Research

Application:

IF:

7.658

Species:

Mus musculus

PMID:

32151678

Title:

Cardamonin retards progression of autosomal dominant polycystic kidney disease via inhibiting renal cyst growth and interstitial fibrosis

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Cell Death Discov

Application:

IF:

5.241

Species:

Danio rerio

PMID:

34001864

Title:

Oxysterol-binding protein-like 2 contributes to the developmental progression of preadipocytes by binding to β-catenin

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

J Transl Med

Application:

IF:

5.531

Species:

Homo sapiens

PMID:

34627268

Title:

CENPA promotes clear cell renal cell carcinoma progression and metastasis via Wnt/β-catenin signaling pathway

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Cellular and molecular life sciences : CMLS

Application:

IF:

9.26

Species:

Homo sapiens

PMID:

33078208

Title:

E2F1-mediated repression of WNT5A expression promotes brain metastasis dependent on the ERK1/2 pathway in EGFR-mutant non-small cell lung cancer

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

WB, IHC

IF:

2.65

Species:

Homo sapiens,Rattus norvegicus

PMID:

35800011

Title:

ShenKang Injection Attenuates Renal Fibrosis by Inhibiting EMT and Regulating the Wnt/β-Catenin Pathway - PMC

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

General and comparative endocrinology

Application:

IF:

3.25

Species:

Capra hircus

PMID:

35300988

Title:

PITX2 regulates steroidogenesis in granulosa cells of dairy goat by the WNT/β-catenin pathway

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Medicine

Application:

IF:

1.81

Species:

Homo sapiens

PMID:

36596012

Title:

Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism - PMC

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Acta Pharmacologica Sinica

Application:

IF:

7.169

Species:

Homo sapiens, Mus musculus

PMID:

31804606

Title:

Ganoderic acid hinders renal fibrosis via suppressing the TGF-/Smad and MAPK signaling pathways

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Pesticide biochemistry and physiology

Application:

IF:

4.96

Species:

Gallus gallus

PMID:

38072524

Title:

miR-181b-1-3p affects the proliferation and differentiation of chondrocytes in TD broilers through the WIF1/Wnt/-catenin pathway

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Biochemical And Biophysical Research Communications

Application:

WB, IF

IF:

3.322

Species:

Rattus norvegicus

PMID:

28365156

Title:

Mild hypothermia protects against oxygen glucose deprivation/reoxygenation-induced apoptosis via the Wnt/-catenin signaling pathway in hippocampal neurons

References for β-Catenin Rabbit pAb (A11512)

Product:

β-Catenin Rabbit pAb

Journal:

Toxics

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38787118

Title:

Integrating Epigenetics, Proteomics, and Metabolomics to Reveal the Involvement of Wnt/-Catenin Signaling Pathway in Oridonin-Induced Reproductive Toxicity - PMC