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Cyclin B1 Rabbit pAb (A2056)

Reviews (7)Publications (38) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cyclin B1 Rabbit pAb (A2056)

Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (A2056) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)

Immunofluorescence analysis of HeLa cells using Cyclin B1 Rabbit pAb (A2056) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)

Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Rabbit pAb (A2056) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)

Immunofluorescence analysis of PC-12 cells using Cyclin B1 Rabbit pAb (A2056) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for Cyclin B1 Rabbit pAb(A2056)
Review
ABclonal: review for Cyclin B1 Rabbit pAb(A2056)
Review
ABclonal: review for Cyclin B1 Rabbit pAb(A2056)
Review
ABclonal: review for Cyclin B1 Rabbit pAb(A2056)
Review
ABclonal: review for Cyclin B1 Rabbit pAb(A2056)
Review
ABclonal: review for Cyclin B1 Rabbit pAb(A2056)
Review
ABclonal: review for Cyclin B1 Rabbit pAb(A2056)

Overview

Product nameCyclin B1 Rabbit pAb
Catalog No.A2056
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 334-433 of human Cyclin B1 (NP_114172.1).
SequenceYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV
Gene ID
Swiss Prot
SynonymsCCNB
Calculated MW48kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesC2C12, C6, Jurkat, Rat testis
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Customer validation

(H460, Human tumor cell, MEF, A172, HeLa, HCT116)

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Cyclin B1 Rabbit pAb (A2056)}

Western blot - Cyclin B1 Rabbit pAb (A2056)

Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (A2056) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)}

Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)

Immunofluorescence analysis of HeLa cells using Cyclin B1 Rabbit pAb (A2056) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)}

Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)

Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Rabbit pAb (A2056) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)}

Immunofluorescence - Cyclin B1 Rabbit pAb (A2056)

Immunofluorescence analysis of PC-12 cells using Cyclin B1 Rabbit pAb (A2056) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

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WBELISA

Reactivity:Human, Mouse

[KO Validated] Cyclin B1 Rabbit mAb

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WBIHC-PIF/ICCIPELISA

Reactivity:Human, Mouse, Rat

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A22435

WBIHC-PIPELISA

Reactivity:Human

Cyclin B1 Rabbit mAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)