제품 > 항체 > 다중클론항체(pAb)

Caspase-9 Rabbit pAb (A0281)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Caspase-9 Rabbit pAb (A0281) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Caspase-9 Rabbit pAb (A0281) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Caspase-9 Rabbit pAb (A0281) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Caspase-9 Rabbit pAb (A0281)

Immunofluorescence analysis of C6 cells using Caspase-9 Rabbit pAb (A0281) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 Rabbit pAb (A0281) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Caspase-9 Rabbit pAb (A0281) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Caspase-9 Rabbit pAb
Catalog No.	A0281
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_001220.2).
Sequence	VGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS
Gene ID
Swiss Prot
Synonyms	MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; Caspase-9
Calculated MW	46kDa
Observed MW	35kDa/37kDa/47kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:100 - 1:500 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HepG2, 293T
Cellular location	apoptosome, cytoplasm, cytosol, mitochondrion, nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Bos taurus, Sanghuangporus vaninii, Rattus norvegicus, Eriocheir sinensis, ovis aries) IHC(Homo sapiens, Gallus gallus)

Documents

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Research Area

Caspase-9 Rabbit pAb images

Immunohistochemistry - Caspase-9 Rabbit pAb (A0281)

Immunofluorescence - Caspase-9 Rabbit pAb (A0281)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Scientific Reports

Application:

IF:

4.379

Species:

Homo sapiens

PMID:

26334131

Title:

FoxM1 Directs STAT3 Expression Essential for Human Endometrial Stromal Decidualization

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

American Journal of Translational Research

Application:

IF:

4.06

Species:

Mus musculus

PMID:

28078005

Title:

Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Frontiers in microbiology

Application:

IF:

5.64

Species:

Bos taurus

PMID:

29670605

Title:

Antiviral Immunotoxin Against Bovine herpesvirus-1: Targeted Inhibition of Viral Replication and Apoptosis of Infected Cell

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Drug Design Development and Therapy

Application:

IF:

4.162

Species:

Homo sapiens

PMID:

31417240

Title:

Phosphocreatine attenuates Gynura segetum-induced hepatocyte apoptosis via a SIRT3-SOD2-mitochondrial reactive oxygen species pathway.

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Int J Biol Macromol

Application:

IF:

6.953

Species:

Sanghuangporus vaninii

PMID:

32629056

Title:

Characterization of a polysaccharide from Sanghuangporus vaninii and its antitumor regulation via activation of the p53 signaling pathway in breast cancer MCF-7 …

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Phytomedicine

Application:

IF:

5.34

Species:

Mus musculus

PMID:

33130473

Title:

Hyperoside ameliorates the progression of osteoarthritis: an in vitro and in vivo study

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Cell Death Dis

Application:

IF:

8.469

Species:

Homo sapiens

PMID:

33441536

Title:

FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Rattus norvegicus

PMID:

34322020

Title:

Selonsertib Alleviates the Progression of Rat Osteoarthritis: An in vitro and in vivo Study

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Journal of virology

Application:

IF:

6.54

Species:

Bos taurus

PMID:

35993735

Title:

Downregulation of the Long Noncoding RNA IALNCR Targeting MAPK8/JNK1 Promotes Apoptosis and Antagonizes Bovine Viral Diarrhea Virus Replication in Host …

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Cancers

Application:

IF:

6.57

Species:

Homo sapiens

PMID:

36900408

Title:

Sodium New Houttuyfonate Induces Apoptosis of Breast Cancer Cells via ROS/PDK1/AKT/GSK3β Axis - PMC

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Mus musculus

PMID:

37071966

Title:

Twist1 Rescue Calcium Overload And Apoptosis Induced by Inflammatory Microenvironment in S. Aureus-Induced Osteomyelitis

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Comp Biochem Physiol C Toxicol Pharmacol

Application:

IF:

4.52

Species:

Eriocheir sinensis

PMID:

34597777

Title:

Protective effects of dietary icariin on lipopolysaccharide-induced acute oxidative stress and hepatopancreas injury in Chinese mitten crab, Eriocheir sinensis

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Homo sapiens

PMID:

37866465

Title:

Neurotoxicity study of ethyl acetate extract of Zanthoxylum armatum DC. on SH-SY5Y based on ROS mediated mitochondrial apoptosis pathway

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Animals : an open access journal from MDPI

Application:

IF:

3.23

Species:

ovis aries

PMID:

38136923

Title:

Resveratrol Improves the Frozen-Thawed Ram Sperm Quality - PMC

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Carbohydrate polymers

Application:

IHC

IF:

10.72

Species:

Homo sapiens

PMID:

38171658

Title:

An oxidized dextran-composite self-healing coated magnesium scaffold reduces apoptosis to induce bone regeneration

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Mus musculus

PMID:

37002200

Title:

Inhibition of TRADD ameliorates chondrocyte necroptosis and osteoarthritis by blocking RIPK1-TAK1 pathway and restoring autophagy - PMC

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Experimental hematology & oncology

Application:

IF:

8.59

Species:

Homo sapiens

PMID:

37679762

Title:

CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression - PMC

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IHC

IF:

7.12

Species:

Gallus gallus

PMID:

37992645

Title:

Baicalin inhibits apoptosis and enhances chondrocyte proliferation in thiram-induced tibial dyschondroplasia in chickens by regulating Bcl-2/Caspase-9 and

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Cancer cell international

Application:

IF:

5.8

Species:

Homo sapiens

PMID:

38769521

Title:

Smad2/3/4 complex could undergo liquid liquid phase separation and induce apoptosis through TAT in hepatocellular carcinoma - PMC

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Frontiers in Pharmacology

Application:

IF:

5.6

Species:

Mus musculus

PMID:

38562460

Title:

Isoquercitrin alleviates pirarubicin-induced cardiotoxicity in vivo and in vitro by inhibiting apoptosis through Phlpp1/AKT/Bcl-2 signaling pathway - PMC

References for Caspase-9 Rabbit pAb (A0281)

Product:

Caspase-9 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.4

Species:

Homo sapiens

PMID:

37884218

Title:

A composition of ursolic acid derivatives from Ludwigia hyssopifolia induces apoptosis in throat cancer cells via the Akt/mTOR and mitochondrial signaling pathways