제품 > 항체 > 단일클론항체(mAb)

Caspase-9 Rabbit mAb (A18676)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Caspase-9 Rabbit mAb (A18676) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Caspase-9 Rabbit mAb (A18676)

Immunofluorescence analysis of C6 cells using Caspase-9 Rabbit mAb (A18676) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Caspase-9 Rabbit mAb (A18676) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Caspase-9 Rabbit mAb
Catalog No.	A18676
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5016-02

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 139-416 of Caspase-9 (NP_001220.2).
Sequence	VGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS
Gene ID
Swiss Prot
Synonyms	MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; Caspase-9
Calculated MW	46kDa
Observed MW	47kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:100 - 1:400 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, Jurkat, Mouse brain, Rat brain
Cellular location	apoptosome, cytoplasm, cytosol, mitochondrion, nucleus.
Customer validation	WB(Gallus gallus, Homo sapiens, Rattus norvegicus) IF(Danio rerio)

Documents

Certificate of Compliance

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Lot number

Research Area

Caspase-9 Rabbit mAb images

Western blot - Caspase-9 Rabbit mAb (A18676)

Immunofluorescence - Caspase-9 Rabbit mAb (A18676)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Caspase-9 Rabbit mAb (A18676)

Product:

Caspase-9 Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Gallus gallus

PMID:

35833119

Title:

Lnc90386 Sponges miR-33-5p to Mediate Mycoplasma gallisepticum-Induced Inflammation and Apoptosis in Chickens via the JNK Pathway - PMC

References for Caspase-9 Rabbit mAb (A18676)

Product:

Caspase-9 Rabbit mAb

Journal:

Toxicology and applied pharmacology

Application:

IF:

4.46

Species:

Danio rerio

PMID:

35093381

Title:

Melatonin alleviates doxorubicin-induced mitochondrial oxidative damage and ferroptosis in cardiomyocytes by regulating YAP expression

References for Caspase-9 Rabbit mAb (A18676)

Product:

Caspase-9 Rabbit mAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Homo sapiens

PMID:

36762489

Title:

Latex Derived from Ficus carica L. Inhibited the Growth of NSCLC by Regulating the Caspase/Gasdermin/AKT Signaling Pathway

References for Caspase-9 Rabbit mAb (A18676)

Product:

Caspase-9 Rabbit mAb

Journal:

International journal of molecular medicine

Application:

IF:

5.31

Species:

Homo sapiens

PMID:

37026504

Title:

Dihydroartemisinin inhibits the activation and proliferation of hepatic stellate cells by regulating miR-29b-3p - PMC

References for Caspase-9 Rabbit mAb (A18676)

Product:

Caspase-9 Rabbit mAb

Journal:

Neuroreport

Application:

IF:

1.7

Species:

Rattus norvegicus

PMID:

37851367

Title:

Total flavonoids of Cynomorium songaricum attenuates cognitive defects in an A1-42-induced Alzheimers disease rat model by activating BDNF/TrkB signaling transduction - PMC

References for Caspase-9 Rabbit mAb (A18676)

Product:

Caspase-9 Rabbit mAb

Journal:

Bioactive materials

Application:

IF:

18.9

Species:

Homo sapiens

PMID:

38440322