Recombinant Human DNMT1 Protein (RP01868LQ)

Overview

Product name	Recombinant Human DNMT1 Protein
Catalog No.	RP01868LQ
Description	Recombinant Human DNMT1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro2-Asp1632) of Human DNMT1 (Accession #NP_001124295.1) fused with a His tag at the C-terminus.
Bio-Activity	The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 µM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 µM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 °C for 1 hr. Then methyltransferase assay was performed using MTase-Glo™ Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent RP01868LQ, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data)
Endotoxin	<1EU/μg
Formulation	Supplied as 0.22 μm filtered solution in 25mM Hepes-NaOH, 500mM NaCl, 0.04%, tritonX 100, 0.5mM TCEP, 10%glycerol, pH7.5.
Species	Human
Calculated MW	185.53 kDa

DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

Product information

Synonym	DNMT1; AIM; CXXC9; DNMT; DNA (cytosine-5)-methyltransferase 1; Dnmt1; 2.1.1.37; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.