제품 > 항체 > 다중클론항체(pAb)

[KD Validated] DNMT1 Rabbit pAb (A16729)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and DNMT1 knockdown (KD) 293T cells using [KD Validated] DNMT1 Rabbit pAb (A16729) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 3 s.

Western blot analysis of lysates from Jurkat cells using [KD Validated] DNMT1 Rabbit pAb (A16729) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 3 s.

ABclonal:Immunohistochemistry - [KD Validated] DNMT1 Rabbit pAb (A16729)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using [KD Validated] DNMT1 Rabbit pAb (A16729) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human tonsil using [KD Validated] DNMT1 Rabbit pAb (A16729) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name	[KD Validated] DNMT1 Rabbit pAb
Catalog No.	A16729
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of DNMT1 (NP_001370.1).
Sequence	MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMAD
Gene ID
Swiss Prot
Synonyms	AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; [KD Validated] DNMT1
Calculated MW	183kDa
Observed MW	200kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293T, Jurkat
Cellular location	Nucleus
Customer validation	WB(Rattus norvegicus, Homo sapiens, Mus musculus, Mus musculus) IF(Mus musculus) IF(Homo sapiens, Mus musculus) ChIP(Mus musculus) ChIP(Mus musculus) Other(Other)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] DNMT1 Rabbit pAb images

Western blot - [KD Validated] DNMT1 Rabbit pAb (A16729)

Immunohistochemistry - [KD Validated] DNMT1 Rabbit pAb (A16729)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

33551813

Title:

Role of GABRD Gene Methylation in the Nucleus Accumbens in Heroin-Seeking Behavior in Rats.

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

Biology open

Application:

IF:

1.8

Species:

Homo sapiens

PMID:

35238333

Title:

DNMT1 is a negative regulator of osteogenesis

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

Cancer letters

Application:

IF:

9.1

Species:

Homo sapiens

PMID:

35131383

Title:

ARID1A loss-of-function induces CpG island methylator phenotype

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

IF:

Species:

Mus musculus

PMID:

36403215

Title:

Disturbed Flow‐Facilitated Margination and Targeting of Nanodisks Protect against Atherosclerosis

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

Protein & cell

Application:

IF:

13.6

Species:

Mus musculus

PMID:

36921016

Title:

The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

eLife

Application:

IF:

6.4

Species:

Mus musculus

PMID:

37929702

Title:

GLI1 facilitates collagen-induced arthritis in mice by collaborative regulation of DNA methyltransferases - PMC

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

Reproduction (Cambridge, England)

Application:

IF:

3.7

Species:

Homo sapiens

PMID:

37590121

Title:

Prenatal cadmium exposure has inter-generational adverse effects on Sertoli cells through the follicle-stimulating hormone receptor pathway

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

Journal of molecular and cellular cardiology

Application:

WB, IF

IF:

4.9

Species:

Homo sapiens, Mus musculus

PMID:

30659837

Title:

DNA methyltransferase 1 and Krppel-like factor 4 axis regulates macrophage inflammation and atherosclerosis.

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Mus musculus

PMID:

36106364

Title:

Geometric Constraints Regulate Energy Metabolism and Cellular Contractility in Vascular Smooth Muscle Cells by Coordinating Mitochondrial DNA Methylation

References for [KD Validated] DNMT1 Rabbit pAb (A16729)

Product:

[KD Validated] DNMT1 Rabbit pAb

Journal:

NPJ Regenerative medicine

Application:

WB, ChIP

IF:

14.4

Species:

Mus musculus

PMID: