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Phospho-DNMT1-S714 Rabbit pAb (AP0021)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - Phospho-DNMT1-S714 Rabbit pAb (AP0021)

Immunofluorescence analysis of MCF-7 cells using Phospho-DNMT1-S714 antibody (AP0021). Blue: DAPI for nuclear staining.

Overview

Product namePhospho-DNMT1-S714 Rabbit pAb
Catalog No.AP0021
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic phosphorylated peptide around S714 of human DNMT1 (NP_001370.1).
SequenceMPSPK
Gene ID
Swiss Prot
SynonymsAIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
Calculated MW183kDa
Observed MWRefer to Figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples
Cellular locationNucleus

Documents

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ABclonal:Immunofluorescence - Phospho-DNMT1-S714 Rabbit pAb (AP0021)}

Immunofluorescence - Phospho-DNMT1-S714 Rabbit pAb (AP0021)

Immunofluorescence analysis of MCF-7 cells using Phospho-DNMT1-S714 antibody (AP0021). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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재조합 단백질 (6)

Secondary Antibodies (26)