Western blot - DNMT1 Rabbit mAb (A22455)
Western blot analysis of various lysates using DNMT1 Rabbit mAb (A22455) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot - DNMT1 Rabbit mAb (A22455)
Western blot analysis of various lysates using DNMT1 Rabbit mAb (A22455) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunohistochemistry - DNMT1 Rabbit mAb (A22455)
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - DNMT1 Rabbit mAb (A22455)
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - DNMT1 Rabbit mAb (A22455)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - DNMT1 Rabbit mAb (A22455)
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - DNMT1 Rabbit mAb (A22455)
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - DNMT1 Rabbit mAb (A22455)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - DNMT1 Rabbit mAb (A22455)
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - DNMT1 Rabbit mAb (A22455)
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation - DNMT1 Rabbit mAb (A22455)
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg DNMT1 antibody (A22455). Western blot was performed from the immunoprecipitate using DNMT1 (A22455) at a dilution of 1:1000.