제품 > 항체 > 아세틸화 특이적 항체

Acetyl-DNMT1-K1127/K1129/K1131/K1133 Rabbit pAb (A5595)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunofluorescence analysis of C6 cells using Acetyl-DNMT1-K1127/K1129/K1131/K1133 Rabbit pAb (A5595) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Acetyl-DNMT1-K1127/K1129/K1131/K1133 Rabbit pAb
Catalog No.	A5595
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic acetylated peptide around K1127 & K1129 & K1131 & K1133 of human DNMT1 (NP_001124295.1).
Sequence	KGKGKGK
Gene ID
Swiss Prot
Synonyms	AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; Acetyl-DNMT1-K1127/K1129/K1131/K1133
Calculated MW	183kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:100 IF/ICC 1:50 - 1:100 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Immunohistochemistry Immunofluorescence
Positive samples
Cellular location	Nucleus.
Customer validation	WB(Mus musculus)