Recombinant Human B7-H1/PD-L1/CD274 Protein (RP02045)

Overview

Product name	Recombinant Human B7-H1/PD-L1/CD274 Protein
Catalog No.	RP02045
Description	Recombinant Human CD30 Ligand/TNFSF8 Protein is produced by mammalian expression system. The target protein is expressed with sequence (Phe19-Arg238) of human PD-L1 (Accession #Q9NZQ7) fused with an Fc tag at the C-terminus.
Bio-Activity	Immobilized Human PD-L1 at 0.2 μg/mL (100 μL/well), dose response curve for Anti-PD-L1 Ab with the EC₅₀ of 1.46 ng/mL determined by ELISA.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	50.9 kDa

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Product information

Synonym	B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1; CD274; PDL1; B7H1; PD-L1; PDCD1L1; PDCD1LG1; B7-H; CD274 molecule
Expression Host	HEK293 cells
Tag	C-mFc
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.