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PD-L1/CD274 Rabbit mAb (A20270)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PD-L1/CD274 Rabbit mAb (A20270)

Flow cytometry: PD-L1/CD274 expressing 293F cells were stained with control IgG or PD-L1/CD274 Rabbit mAb (A20270).
Primary:PD-L1/CD274 Rabbit mAb (A20270) at 1:100 dilution
Secondary:1:1000 goat anti-rabbit IgG FITC

ABclonal:Inhibition - PD-L1/CD274 Rabbit mAb (A20270)

1μg/mL of biotinylated PD-L1/CD274 protein was incubated with PD-L1/CD274 Rabbit mAb (A20270) or control mAb with serial dilution for 1 hour at RT. The mixture was added to ELISA plate coated with 1μg/mL of recombinant expressed PD-1. HRP-Streptavidin was used for biotinylated PD-L1/CD274 detection.

Overview

Product namePD-L1/CD274 Rabbit mAb
Catalog No.A20270
Host speciesRabbit
Purification method≥95% purity by SDS-PAGE
IsotypeIgG
CloneNo.ARC5128-01
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PD-L1/CD274 (Q9NZQ7)
SequenceMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET
Gene ID
Swiss Prot
SynonymsB7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1; PD-L1/CD274
Calculated MW33kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 1:50 - 1:200
  • Elisa 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Endomembrane system, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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ABclonal:Flow CytoMetry - PD-L1/CD274 Rabbit mAb (A20270)}

Flow CytoMetry - PD-L1/CD274 Rabbit mAb (A20270)

Flow cytometry: PD-L1/CD274 expressing 293F cells were stained with control IgG or PD-L1/CD274 Rabbit mAb (A20270).
Primary:PD-L1/CD274 Rabbit mAb (A20270) at 1:100 dilution
Secondary:1:1000 goat anti-rabbit IgG FITC
ABclonal:Inhibition - PD-L1/CD274 Rabbit mAb (A20270)}

Inhibition - PD-L1/CD274 Rabbit mAb (A20270)

1μg/mL of biotinylated PD-L1/CD274 protein was incubated with PD-L1/CD274 Rabbit mAb (A20270) or control mAb with serial dilution for 1 hour at RT. The mixture was added to ELISA plate coated with 1μg/mL of recombinant expressed PD-1. HRP-Streptavidin was used for biotinylated PD-L1/CD274 detection.

* For research use only. Not for therapeutic or diagnostic purposes.

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