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ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and MDA-MB-231 cells (right) were surface-stained with ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880)

Flow cytometry: 1X10^6 MDA-MB-231 cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880, 5 μl/Test, right).

Overview

Product nameABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb
Catalog No.A25880
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5128-01-ABf450
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PD-L1/CD274 (Q9NZQ7
SequenceMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET
Gene ID
Swiss Prot
SynonymsB7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
Calculated MW33kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationactin cytoskeleton, external side of plasma membrane, extracellular exosome, nucleoplasm, plasma membrane
ABclonal:Flow CytoMetry - ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880)}

Flow CytoMetry - ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and MDA-MB-231 cells (right) were surface-stained with ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880)}

Flow CytoMetry - ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880)

Flow cytometry: 1X10^6 MDA-MB-231 cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human PD-L1/CD274 mAb (A25880, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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