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Recombinant Human B7-H1/PD-L1/CD274 Protein (RP00331)

Datasheet

Overview

Product nameRecombinant Human B7-H1/PD-L1/CD274 Protein
Catalog No.RP00331
DescriptionRecombinant Human PD-L1/B7-H1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe19-Arg238) of Human PD-L1/B7-H1 (Accession #Q9NZQ7-1) fused with a C-hFc tag at the C-terminus.
Purity> 95% by SDS-PAGE;> 95% by HPLC
Endotoxin< 1 EU/μg of the protein by LAL method
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
SpeciesHuman
Calculated MW52 kDa
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
SynonymCD274; PDL1; PD-L1; PD-L1B7 homolog 1; B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Expression HostHEK293 cells
TagC-hFc
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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