Product name | Recombinant Human B7-H1/PD-L1/CD274 Protein |
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Catalog No. | RP00068 |
Description | Recombinant Human B7-H1/PD-L1/CD274 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Phe19-Thr239) of human PD-L1/B7-H1 (Accession #NP_054862.1) fused with a 6×His tag at the C-terminus. |
Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human PD-L1 at 10 μg/mL (100 μL/well) can bind Recombinant Human PD-1 with a linear range of 0.25-1.02 μg/mL. 2.Measured by its binding ability in a functional ELISA.Immobilized PE anti-human CD274 (B7-H1, PD-L1) Antibody at 1μg/mL (25 μL/well) can bind Human CD274 with a linear range of 0.46-18ng/mL. |
Purity | > 97% by SDS-PAGE. |
Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Species | Human |
Calculated MW | 26.14 kDa |
Synonym | B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1; CD274; PDL1; B7H1; PD-L1; PDCD1L1; PDCD1LG1; B7-H; CD274 molecule |
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Expression Host | HEK293 cells |
Tag | C-His |
Swiss-Prot | |
Gene ID |
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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