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[KO Validated] PD-L1/CD274 Rabbit PolymAb® (A23136PM)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KO Validated] PD-L1/CD274 Rabbit PolymAb® (A23136PM)

Western blot analysis of lysates from wild type (WT) and PD-L1/CD274 knockout (KO) A549 cells using [KO Validated] PD-L1/CD274 Rabbit PolymAb® (A23136PM) at 1:1000 dilution. wild type (WT) and PD-L1/CD274 knockout (KO) A549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name[KO Validated] PD-L1/CD274 Rabbit PolymAb®
Catalog No.A23136PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human PD-L1/CD274.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsB7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
Calculated MW33kDa
Observed MW40-50kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA549 treated by IFNG
Cellular locationCell membrane, Endomembrane system, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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ABclonal:Western blot - [KO Validated] PD-L1/CD274 Rabbit PolymAb® (A23136PM)}

Western blot - [KO Validated] PD-L1/CD274 Rabbit PolymAb® (A23136PM)

Western blot analysis of lysates from wild type (WT) and PD-L1/CD274 knockout (KO) A549 cells using [KO Validated] PD-L1/CD274 Rabbit PolymAb® (A23136PM) at 1:1000 dilution. wild type (WT) and PD-L1/CD274 knockout (KO) A549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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