제품 > 항체 > 단일클론항체(mAb)

Sarm1 Mouse mAb (A23440)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Sarm1 Mouse mAb (A23440)

Western blot analysis of various lysates, using Sarm1 Mouse mAb (A23440) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameSarm1 Mouse mAb
Catalog No.A23440
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0559
Enables NAD+ nucleosidase activity. Involved in NAD catabolic process; positive regulation of neuron death; and regulation of dendrite morphogenesis. Acts upstream of or within regulation of apoptotic process and response to glucose. Located in several cellular components, including dendrite; microtubule; and mitochondrion. Is extrinsic component of mitochondrial outer membrane. Is expressed in immune system; nervous system; neural retina; and tongue. Orthologous to human SARM1 (sterile alpha and TIR motif containing 1).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse Sarm1 (NP_766383.2).
SequenceMVLTLLFSAYKLCRFFTMSGPRPGADRLTVPGPDRSGGASPWWAAGGRGSREVSPGVGTEVQGALERSLPELQQALSELKQASAARAVGAGLAEVFQLVE
Gene ID
Swiss Prot
SynonymsSarm; MyD885; A830091I15Rik; Sarm1
Calculated MW79kDa
Observed MW72kDa/80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:4000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRaji, Mouse brain, Rat brain
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Sarm1 Mouse mAb (A23440)}

    Western blot - Sarm1 Mouse mAb (A23440)

    Western blot analysis of various lysates, using Sarm1 Mouse mAb (A23440) at 1:4000 dilution.
    Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.