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TDP-43/TARDB Mouse mAb (A25125)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TDP-43/TARDB Mouse mAb (A25125)

Western blot analysis of various lysates using TDP-43/TARDB Mouse mAb (A25125) at 1:800 dilution.
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameTDP-43/TARDB Mouse mAb
Catalog No.A25125
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
CloneNo.AMC0596
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1- 414 of human TDP-43/TARDB (Q13148).
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsALS10; TDP-43; TDP-43/TARDB
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, K-562, Jurkat, Mouse brain, Mouse liver, Rat brain, Rat thymus
Cellular locationNucleus
Customer validation

IF(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - TDP-43/TARDB Mouse mAb (A25125)}

Western blot - TDP-43/TARDB Mouse mAb (A25125)

Western blot analysis of various lysates using TDP-43/TARDB Mouse mAb (A25125) at 1:800 dilution.
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

ELISA 키트 (1)

Secondary Antibodies (22)