Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1 μg TriMethyl-Histone H3-K27 Mouse mAb antibody (A16199) , along with a Goat Anti-Mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K27 in representative gene loci (MYOD1), as shown in figure.
Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A16199) at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 Mouse mAb (A16199) at dilution of 1:25. Blue: DAPI for nuclear staining.
| Product name | TriMethyl-Histone H3-K27 Mouse mAb |
|---|---|
| Catalog No. | A16199 |
| Host species | Mouse |
| Purification method | Affinity purification |
| Isotype | IgG1, kappa |
| CloneNo. | AMC0015 |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
|---|---|
| Sequence | Email for sequence |
| Gene ID | |
| Swiss Prot | |
| Synonyms | H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K27 |
| Calculated MW | 16kDa |
| Observed MW | 17kDa |
| Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HeLa, NIH/3T3, C6, H3 protein |
| Cellular location | Chromosome, Nucleus. |
| Customer validation | WB(Mus musculus, Sus scrofa, Homo sapiens, Mus musculus) IF(Rattus norvegicus, R. canina, R. rubiginosa) ChIP(Oryza sativa) ChIP(Mus musculus) IP(Mus musculus) WB(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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