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cGAS Mouse mAb (A27100)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - cGAS Mouse mAb (A27100)

Western blot analysis of various lysates using cGAS Mouse mAb (A27100) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - cGAS Mouse mAb (A27100)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using cGAS Mouse mAb (A27100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - cGAS Mouse mAb (A27100)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using cGAS Mouse mAb (A27100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - cGAS Mouse mAb (A27100)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using cGAS Mouse mAb (A27100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product namecGAS Mouse mAb
Catalog No.A27100
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2a κ
CloneNo.AMC0693
Enables several functions, including 2', 3'-cyclic GMP-AMP synthase activity; chromatin binding activity; and phosphatidylinositol-4, 5-bisphosphate binding activity. Involved in several processes, including cellular response to exogenous dsRNA; positive regulation of intracellular signal transduction; and regulation of defense response. Located in several cellular components, including cytosol; nucleus; and site of double-strand break.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human cGAS (Q8N884).
SequenceMQPWHGKAMQRASEAGATAPKASARNARGAPMDPTESPAAPEAALPKAGKFGPARKSGSRQKKSAPDTQERPPVRATGARAKKAPQRAQDTQPSDATSAP
Gene ID
Swiss Prot
SynonymsMB21D1; h-cGAS; C6orf150
Calculated MW59kDa
Observed MW63kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.1% Sodium azide, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRT4, RAW 264.7
Cellular locationCytoplasm, cytosol.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - cGAS Mouse mAb (A27100)}

    Western blot - cGAS Mouse mAb (A27100)

    Western blot analysis of various lysates using cGAS Mouse mAb (A27100) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Immunohistochemistry - cGAS Mouse mAb (A27100)}

    Immunohistochemistry - cGAS Mouse mAb (A27100)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using cGAS Mouse mAb (A27100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - cGAS Mouse mAb (A27100)}

    Immunohistochemistry - cGAS Mouse mAb (A27100)

    Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using cGAS Mouse mAb (A27100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - cGAS Mouse mAb (A27100)}

    Immunohistochemistry - cGAS Mouse mAb (A27100)

    Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using cGAS Mouse mAb (A27100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.