제품 > 항체 > 단일클론항체(mAb)

ATPB Mouse mAb (A26922)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATPB Mouse mAb (A26922)

Western blot analysis of various lysates using ATPB Mouse mAb (A26922) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - ATPB Mouse mAb (A26922)

Confocal imaging of PC-12 cells using ATPB Mouse mAb (A26922, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - ATPB Mouse mAb (A26922)

Confocal imaging of Hep G2 cells using ATPB Mouse mAb (A26922, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - ATPB Mouse mAb (A26922)

Confocal imaging of HeLa cells using ATPB Mouse mAb (A26922, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameATPB Mouse mAb
Catalog No.A26922
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
CloneNo.AMC50034
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
ImmunogenA synthesized peptide derived from human ATPB.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsATP5B; ATPMB; ATPSB; HUMOP2; HEL-S-271
Calculated MW57kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:20000
  • IHC-P 1:2000 - 1:20000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Hep G2, Jurkat, Mouse liver, Rat liver, Rat brain
Cellular locationMitochondrion, Mitochondrion inner membrane.

Documents

Certificate of Compliance

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ABclonal:Western blot - ATPB Mouse mAb (A26922)}

Western blot - ATPB Mouse mAb (A26922)

Western blot analysis of various lysates using ATPB Mouse mAb (A26922) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)}

Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)}

Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)}

Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)}

Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ATPB Mouse mAb (A26922)}

Immunohistochemistry - ATPB Mouse mAb (A26922)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using ATPB Mouse mAb (A26922) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - ATPB Mouse mAb (A26922)}

Immunofluorescence - ATPB Mouse mAb (A26922)

Confocal imaging of PC-12 cells using ATPB Mouse mAb (A26922, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - ATPB Mouse mAb (A26922)}

Immunofluorescence - ATPB Mouse mAb (A26922)

Confocal imaging of Hep G2 cells using ATPB Mouse mAb (A26922, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - ATPB Mouse mAb (A26922)}

Immunofluorescence - ATPB Mouse mAb (A26922)

Confocal imaging of HeLa cells using ATPB Mouse mAb (A26922, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.