제품 > 항체 > 단일클론항체(mAb)

CYP7A1 Mouse mAb (A22897)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from Mouse liver, using CYP7A1 Mouse mAb (A22897) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product name	CYP7A1 Mouse mAb
Catalog No.	A22897
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG1 κ
CloneNo.	AMC0581

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 86-108 of human CYP7A1 (P22680).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	CP7A; CYP7; CYPVII; CYP7A1
Calculated MW	58kDa
Observed MW	58kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.1% sodium azide and 0.1% gelatin.
Key application	Western blotting
Positive samples	Mouse liver
Cellular location	Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein.
Customer validation	WB(Rattus norvegicus)