제품 > 항체 > 단일클론항체(mAb)

Fyn Mouse mAb (A0086)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Fyn Mouse mAb (A0086) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Fyn Mouse mAb
Catalog No.	A0086
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Immunogen information

Immunogen	Recombinant protein of human Fyn
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	SLK; SYN; p59-FYN; Fyn
Calculated MW	61kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:200 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, Jurkat, SW620, HT-1080, Mouse lung, Mouse heart, Rat testis
Cellular location	Cell membrane, Cytoplasm, Nucleus
Customer validation	WB(Homo sapiens, Mus musculus, Mus musculus )