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Phospho-Fyn-S21 Rabbit pAb (AP0510)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from A-431 cells, using Phospho-Fyn-S21 Rabbit pAb (AP0510) at 1:1000 dilution. A-431 cells were treated by 10% FBS after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product name	Phospho-Fyn-S21 Rabbit pAb
Catalog No.	AP0510
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S21 of human Phospho-Fyn-S21 (NP_002028.1).
Sequence	DGSLN
Gene ID
Swiss Prot
Synonyms	SLK; SYN; p59-FYN; Phospho-Fyn-S21
Calculated MW	61kDa
Observed MW	60kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431 treated by 10% FBS
Cellular location	Cell membrane, Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus) IHC(Homo sapiens)